Population studies of intact vitamin D binding protein by affinity capture ESI-TOF-MS

Chad Borges, Jason W. Jarvis, Paul E. Oran, Stephen P. Rogers, Randall W. Nelson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Blood plasma proteins with molecular weights greater than approximately 30 kDa are refractory to comprehensive, high-throughput qualitative characterization of microheterogeneity across human populations. Analytical techniques for obtaining high mass resolution for targeted, intact protein characterization and, separately, high sample throughput exist, but efficient means of coupling these assay characteristics remain rather limited. This article discusses the impetus for analyzing intact proteins in a targeted manner across populations and describes the methodology required to couple mass spectrometric immunoassay with electrospray ionization mass spectrometry for the purpose of qualitatively characterizing a prototypical large plasma protein, vitamin D binding protein, across populations.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalJournal of Biomolecular Techniques
Volume19
Issue number3
StatePublished - Jul 1 2008

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Keywords

  • Electrospray ionization
  • Mass spectrometric immunoassay
  • Microheterogeneity
  • Vitamin D binding protein

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Borges, C., Jarvis, J. W., Oran, P. E., Rogers, S. P., & Nelson, R. W. (2008). Population studies of intact vitamin D binding protein by affinity capture ESI-TOF-MS. Journal of Biomolecular Techniques, 19(3), 167-176.