Population structure in the roundtail chub (gila robusta complex) of the gila river basin as determined by microsatellites: Evolutionary and conservation implications

Thomas E. Dowling, Corey D. Anderson, Paul C. Marsh, Michael S. Rosenberg

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ten microsatellite loci were characterized for 34 locations from roundtail chub (Gila robusta complex) to better resolve patterns of genetic variation among local populations in the lower Colorado River basin. This group has had a complex taxonomic history and previous molecular analyses failed to identify species diagnostic molecular markers. Our results supported previous molecular studies based on allozymes and DNA sequences, which found that most genetic variance was explained by differences among local populations. Samples from most localities were so divergent species-level diagnostic markers were not found. Some geographic samples were discordant with current taxonomy due to admixture or misidentification; therefore, additional morphological studies are necessary. Differences in spatial genetic structure were consistent with differences in connectivity of stream habitats, with the typically mainstem species, G. robusta, exhibiting greater genetic connectedness within the Gila River drainage. No species exhibited strong isolation by distance over the entire stream network, but the two species typically found in headwaters, G. nigra and G. intermedia, exhibited greater than expected genetic similarity between geographically proximate populations, and usually clustered with individuals from the same geographic location and/ or sub-basin. These results highlight the significance of microevolutionary processes and importance of maintaining local populations to maximize evolutionary potential for this complex. Augmentation stocking as a conservation management strategy should only occur under extreme circumstances, and potential source populations should be geographically proximate stocks of the same species, especially for the headwater forms.

Original languageEnglish (US)
Article numbere0139832
JournalPLoS One
Volume10
Issue number10
DOIs
StatePublished - Oct 16 2015
Externally publishedYes

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Cyprinidae
Rivers
Catchments
Microsatellite Repeats
Conservation
population structure
microsatellite repeats
DNA sequences
Taxonomies
Population
Isoenzymes
Drainage
Geographic Locations
Colorado River
Molecular Pathology
Genetic Structures
genetic variance
allozymes
Ecosystem
drainage

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Population structure in the roundtail chub (gila robusta complex) of the gila river basin as determined by microsatellites : Evolutionary and conservation implications. / Dowling, Thomas E.; Anderson, Corey D.; Marsh, Paul C.; Rosenberg, Michael S.

In: PLoS One, Vol. 10, No. 10, e0139832, 16.10.2015.

Research output: Contribution to journalArticle

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