Population genetics of the California National Primate Research Center’s (CNPRC) captive Callicebus cupreus colony

Adrian Mendoza, Jillian Ng, Karen L. Bales, Sally P. Mendoza, Debra A. George, David Glenn Smith, Sreetharan Kanthaswamy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The California National Primate Research Center maintains a small colony of titi monkeys (Callicebus cupreus) for behavioral studies. While short tandem repeat (STR) markers are critical for the genetic management of the center’s rhesus macaque (Macaca mulatta) breeding colony, STRs are not used for this purpose in the maintenance of the center’s titi monkey colony. Consequently, the genetic structure of this titi monkey population has not been characterized. A lack of highly informative genetic markers in titi monkeys has also resulted in scant knowledge of the species’ genetic variation in the wild. The purpose of this study was to develop a panel of highly polymorphic titi monkey STRs using a cross-species polymerase chain reaction (PCR) amplification protocol that could be used for the genetic management of the titi monkey colony. We screened 16 STR primer pairs and selected those that generated robust and reproducible polymorphic amplicons. Loci that were found to be highly polymorphic, very likely to be useful for parentage verification, pedigree assessment, and studying titi monkey population genetics, were validated using Hardy–Weinberg equilibrium and linkage disequilibrium analyses. The genetic data generated in this study were also used to assess directly the impact on the colony’s genetic diversity of a recent adenovirus outbreak. While the adenovirus epizootic disease caused significant mortality (19 deaths among the 65 colony animals), our results suggest that the disease exhibited little or no influence on the overall genetic diversity of the colony.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalPrimates
Volume56
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

monkeys
population genetics
Primates
Adenoviridae
Macaca mulatta
genetic variation
microsatellite repeats
parentage
linkage disequilibrium
pedigree
polymerase chain reaction
death
loci
genetic markers
breeding
animals

Keywords

  • Captive populations
  • Closed colony
  • Cross-species amplification
  • Genetic management
  • Paternity
  • Population genetic structure
  • Red or coppery titi monkey

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Population genetics of the California National Primate Research Center’s (CNPRC) captive Callicebus cupreus colony. / Mendoza, Adrian; Ng, Jillian; Bales, Karen L.; Mendoza, Sally P.; George, Debra A.; Smith, David Glenn; Kanthaswamy, Sreetharan.

In: Primates, Vol. 56, No. 1, 2014, p. 37-44.

Research output: Contribution to journalArticle

Mendoza, Adrian ; Ng, Jillian ; Bales, Karen L. ; Mendoza, Sally P. ; George, Debra A. ; Smith, David Glenn ; Kanthaswamy, Sreetharan. / Population genetics of the California National Primate Research Center’s (CNPRC) captive Callicebus cupreus colony. In: Primates. 2014 ; Vol. 56, No. 1. pp. 37-44.
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