Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding protein

K. Satoh, H. Y. Nakatani, K. E. Steinback, J. Watson, C. J. Arntzen

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The polypeptide composition of a Photosystem II (PS II) core complex from higher plant chloroplasts has been characterized by subjecting the isolated complex to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two polypeptides in the 40-50 kDa size class, attributed to the chlorophyll a-binding apoproteins of PS II, were resolved when the urea concentration in the SDS-polyacrylamide gel electrophoresis was greater than 1 M. The two chlorophyll a-binding proteins were dissimilar in their primary structure based upon their different hydrolysis products on SDS-polyacrylamide gel electrophoresis following papain treatment. The core complex contained three additional polypeptides. Two polypeptides in the 30-34 kDa size class were resolved when the urea concentration in the gel system was increased to greater than 4 M. One of the polypeptides in this size class was identified as the herbicide-binding protein from azido[14C]atrazine labeling studies. The herbicide-binding protein displayed an anomalous electrophoretic migration behavior in SDS-polyacrylamide gel electrophoresis in the presence or absence of urea; its apparent molecular weight decreased when the urea concentration increased. The fifth protein component of the core complex was attributed to cytochrome b-559 which was found to consist of the ascorbate- and dithionite-reducible forms in the samples prior to SDS solubilization.

Original languageEnglish (US)
Pages (from-to)142-150
Number of pages9
JournalBBA - Bioenergetics
Volume724
Issue number1
DOIs
StatePublished - Jul 29 1983
Externally publishedYes

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Photosystem II Protein Complex
Herbicides
Carrier Proteins
Electrophoresis
Urea
Polyacrylamide Gel Electrophoresis
Peptides
Chemical analysis
Chlorophyll Binding Proteins
Dithionite
Atrazine
Apoproteins
Papain
Chloroplasts
Sodium Dodecyl Sulfate
Labeling
Hydrolysis
Molecular Weight
Gels
Molecular weight

Keywords

  • Atrazine
  • Chlorophyll fluorescence
  • Herbicide-binding protein
  • Photosystem II
  • Polypeptide composition

ASJC Scopus subject areas

  • Biophysics

Cite this

Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding protein. / Satoh, K.; Nakatani, H. Y.; Steinback, K. E.; Watson, J.; Arntzen, C. J.

In: BBA - Bioenergetics, Vol. 724, No. 1, 29.07.1983, p. 142-150.

Research output: Contribution to journalArticle

Satoh, K. ; Nakatani, H. Y. ; Steinback, K. E. ; Watson, J. ; Arntzen, C. J. / Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding protein. In: BBA - Bioenergetics. 1983 ; Vol. 724, No. 1. pp. 142-150.
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