Polymorphism of the Pv200L fragment of merozoite surface protein-1 of Plasmodium vivax in clinical isolates from the Pacific Coast of Colombia

Augusto Valderrama-Aguirre, Evelin Zúñiga-Soto, Leonardo Mariño-Ramírez, Luz Ángela Moreno, Ananías A. Escalante, Myriam Arévalo-Herrera, Sócrates Herrera

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Abstract

Merozoite surface protein 1 (MSP-1) is a polymorphic malaria protein with functional domains involved in parasite erythrocyte interaction. Plasmodium vivax MSP-1 has a fragment ( Pv200L) that has been identified as a potential subunit vaccine because it is highly immunogenic and induces partial protection against infectious parasite challenge in vaccinated monkeys. To determine the extent of genetic polymorphism and its effect on the translated protein, we sequenced the Pv200L coding region from isolates of 26 P. vivax-infected patients in a malaria-endemic area of Colombia. The extent of nucleotide diversity (π) in these isolates (0.061 ± 0.004) was significantly lower (P ≤ 0.001) than that observed in Thai and Brazilian isolates; 0.083 ± 0.006 and 0.090 ± 0.006, respectively. We found two new alleles and several previously unidentified dimorphic substitutions and significant size polymorphism. The presence of highly conserved blocks in this fragment has important implications for the development of Pv200L as a subunit vaccine candidate.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume84
Issue number2 S
DOIs
StatePublished - Feb 1 2011

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ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Cite this

Valderrama-Aguirre, A., Zúñiga-Soto, E., Mariño-Ramírez, L., Moreno, L. Á., Escalante, A. A., Arévalo-Herrera, M., & Herrera, S. (2011). Polymorphism of the Pv200L fragment of merozoite surface protein-1 of Plasmodium vivax in clinical isolates from the Pacific Coast of Colombia. American Journal of Tropical Medicine and Hygiene, 84(2 S), 64-70. https://doi.org/10.4269/ajtmh.2011.09-0517