Polymerization-based signal amplification for paper-based immunoassays

Abraham K. Badu-Tawiah, Shefali Lathwal, Kaja Kaastrup, Mohammad Al-Sayah, Dionysios C. Christodouleas, Barbara Smith, George M. Whitesides, Hadley D. Sikes

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Diagnostic tests in resource-limited settings require technologies that are affordable and easy to use with minimal infrastructure. Colorimetric detection methods that produce results that are readable by eye, without reliance on specialized and expensive equipment, have great utility in these settings. We report a colorimetric method that integrates a paper-based immunoassay with a rapid, visible-light-induced polymerization to provide high visual contrast between a positive and a negative result. Using Plasmodium falciparum histidine-rich protein 2 as an example, we demonstrate that this method allows visual detection of proteins in complex matrices such as human serum and provides quantitative information regarding analyte levels when combined with cellphone-based imaging. It also allows the user to decouple the capture of analyte from signal amplification and visualization steps.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number3
DOIs
StatePublished - Feb 7 2015
Externally publishedYes

Fingerprint

Immunoassay
Polymerization
Amplification
Visualization
Proteins
Imaging techniques
Plasmodium falciparum
Routine Diagnostic Tests
Technology
Light
Equipment and Supplies
Serum
histidine-rich proteins

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Badu-Tawiah, A. K., Lathwal, S., Kaastrup, K., Al-Sayah, M., Christodouleas, D. C., Smith, B., ... Sikes, H. D. (2015). Polymerization-based signal amplification for paper-based immunoassays. Lab on a Chip - Miniaturisation for Chemistry and Biology, 15(3), 655-659. https://doi.org/10.1039/c4lc01239a

Polymerization-based signal amplification for paper-based immunoassays. / Badu-Tawiah, Abraham K.; Lathwal, Shefali; Kaastrup, Kaja; Al-Sayah, Mohammad; Christodouleas, Dionysios C.; Smith, Barbara; Whitesides, George M.; Sikes, Hadley D.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 15, No. 3, 07.02.2015, p. 655-659.

Research output: Contribution to journalArticle

Badu-Tawiah, AK, Lathwal, S, Kaastrup, K, Al-Sayah, M, Christodouleas, DC, Smith, B, Whitesides, GM & Sikes, HD 2015, 'Polymerization-based signal amplification for paper-based immunoassays', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 15, no. 3, pp. 655-659. https://doi.org/10.1039/c4lc01239a
Badu-Tawiah, Abraham K. ; Lathwal, Shefali ; Kaastrup, Kaja ; Al-Sayah, Mohammad ; Christodouleas, Dionysios C. ; Smith, Barbara ; Whitesides, George M. ; Sikes, Hadley D. / Polymerization-based signal amplification for paper-based immunoassays. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2015 ; Vol. 15, No. 3. pp. 655-659.
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