TY - JOUR
T1 - Polymer coating of copper oxide nanoparticles increases nanoparticles uptake and toxicity in the green alga Chlamydomonas reinhardtii
AU - Perreault, François
AU - Oukarroum, Abdallah
AU - Melegari, Silvia Pedroso
AU - Matias, William Gerson
AU - Popovic, Radovan
N1 - Funding Information:
This work was supported by research grants awarded to R. Popovic by the Natural Sciences and Engineering Research Council of Canada (NSERC, Canada). F. Perreault is supported by a NSERC Ph.D fellowship. S.P. Melegari was supported by a Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) post-doctoral fellowship. The authors thank Pr. Jérôme Claverie (UQAM) for providing the CS-CuO NPs.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2012/6
Y1 - 2012/6
N2 - Copper oxide nanoparticles (CuO NPs) are frequently used in a polymer-coated form, to be included in paints or fabrics for antimicrobial properties. Their application in antifouling paints may lead to the contamination of aquatic ecosystems. However, the toxicological risk of NPs in the environment is hard to evaluate due to a lack of knowledge on the mechanisms of NP interaction with biological systems. In this study, we investigated the effect of polymer coating on CuO NP toxicity in the green alga Chlamydomonas reinhardtii by comparing bare and polymer-coated CuO NPs prepared from the same CuO nanopowder. Both CuO NP suspensions were toxic to C. reinhardtii after 6h treatment to concentrations of 0.005-0.04gL-1. Bare and polymer-coated CuO NPs induced a decrease of Photosystem II activity and the formation of reactive oxygen species. Polymer-coated CuO NP was found to be more toxic than the uncoated CuO NP. The higher toxicity of CS-CuO NP was mainly associated with the increased capacity of polymer-coated CuO NP to penetrate the cell compared to bare CuO NPs. These results indicates that the high toxicity of polymer-coated CuO NPs in algal cells results of intracellular interactions between NPs and the cellular system.
AB - Copper oxide nanoparticles (CuO NPs) are frequently used in a polymer-coated form, to be included in paints or fabrics for antimicrobial properties. Their application in antifouling paints may lead to the contamination of aquatic ecosystems. However, the toxicological risk of NPs in the environment is hard to evaluate due to a lack of knowledge on the mechanisms of NP interaction with biological systems. In this study, we investigated the effect of polymer coating on CuO NP toxicity in the green alga Chlamydomonas reinhardtii by comparing bare and polymer-coated CuO NPs prepared from the same CuO nanopowder. Both CuO NP suspensions were toxic to C. reinhardtii after 6h treatment to concentrations of 0.005-0.04gL-1. Bare and polymer-coated CuO NPs induced a decrease of Photosystem II activity and the formation of reactive oxygen species. Polymer-coated CuO NP was found to be more toxic than the uncoated CuO NP. The higher toxicity of CS-CuO NP was mainly associated with the increased capacity of polymer-coated CuO NP to penetrate the cell compared to bare CuO NPs. These results indicates that the high toxicity of polymer-coated CuO NPs in algal cells results of intracellular interactions between NPs and the cellular system.
KW - Chlamydomonas reinhardtii
KW - Copper oxide nanoparticle
KW - Nanotoxicology
KW - Photosynthesis
KW - Reactive oxygen species
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U2 - 10.1016/j.chemosphere.2012.02.046
DO - 10.1016/j.chemosphere.2012.02.046
M3 - Article
C2 - 22445953
AN - SCOPUS:84859499603
SN - 0045-6535
VL - 87
SP - 1388
EP - 1394
JO - Chemosphere
JF - Chemosphere
IS - 11
ER -