Polyelectrolyte platform for sensitive detection of biological analytes via reversible fluorescence quenching

Michael J. O'Connell; Candace K. Chan; Wenguang Li; Raea K. Hicks; Stephen K. Doorn; Hsing Lin Wang

doi:10.1016/j.polymer.2007.10.036

Polyelectrolyte platform for sensitive detection of biological analytes via reversible fluorescence quenching

Michael J. O'Connell, Candace K. Chan, Wenguang Li, Raea K. Hicks, Stephen K. Doorn, Hsing Lin Wang

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Non-conjugated polyelectrolytes were used to quench oppositely charged fluorescent dye-ligand conjugate (DLC) molecules by bringing them to the vicinity of the polyelectrolyte backbone to form aggregates and hence quench the dye fluorescence. As target protein molecules are added to the solution, the specific interaction between the DLC and the protein disrupts the aggregate structure, thus recovering the luminescence. The binding of DLC to oppositely charged polyelectrolyte and the disruption of the aggregates are investigated by fluorescence spectroscopy. The static-quenching mechanism is clearly manifested in the Stern-Volmer plots that show the decrease in slope with increasing temperature. This polyelectrolyte-based sensing platform has a sensitivity of <100 pM. We also show the selectivity of this platform by comparing the fluorescence recovery between two proteins (avidin and bovine serum albumin) with similar molecular weight. Our results suggest a highly sensitive approach for detecting biological analytes.

Original language	English (US)
Pages (from-to)	7582-7589
Number of pages	8
Journal	Polymer
Volume	48
Issue number	26
DOIs	https://doi.org/10.1016/j.polymer.2007.10.036
State	Published - Dec 13 2007
Externally published	Yes

Keywords

Electrostatic interaction
Fluorescence
Polyelectrolyte

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1016/j.polymer.2007.10.036

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@article{ad8e152329474a58bf8325587bdedd6e,

title = "Polyelectrolyte platform for sensitive detection of biological analytes via reversible fluorescence quenching",

abstract = "Non-conjugated polyelectrolytes were used to quench oppositely charged fluorescent dye-ligand conjugate (DLC) molecules by bringing them to the vicinity of the polyelectrolyte backbone to form aggregates and hence quench the dye fluorescence. As target protein molecules are added to the solution, the specific interaction between the DLC and the protein disrupts the aggregate structure, thus recovering the luminescence. The binding of DLC to oppositely charged polyelectrolyte and the disruption of the aggregates are investigated by fluorescence spectroscopy. The static-quenching mechanism is clearly manifested in the Stern-Volmer plots that show the decrease in slope with increasing temperature. This polyelectrolyte-based sensing platform has a sensitivity of <100 pM. We also show the selectivity of this platform by comparing the fluorescence recovery between two proteins (avidin and bovine serum albumin) with similar molecular weight. Our results suggest a highly sensitive approach for detecting biological analytes.",

keywords = "Electrostatic interaction, Fluorescence, Polyelectrolyte",

author = "O'Connell, {Michael J.} and Chan, {Candace K.} and Wenguang Li and Hicks, {Raea K.} and Doorn, {Stephen K.} and Wang, {Hsing Lin}",

note = "Funding Information: This work is supported by the LDRD program of Los Alamos National Laboratory, the Office of Science (DOE), and Cross Enterprise Technology Development Program from National Aeronautics and Space Administration, NASA. MJO acknowledges support from the DCI postdoctoral fellowship.",

year = "2007",

month = dec,

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doi = "10.1016/j.polymer.2007.10.036",

language = "English (US)",

volume = "48",

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T1 - Polyelectrolyte platform for sensitive detection of biological analytes via reversible fluorescence quenching

AU - O'Connell, Michael J.

AU - Chan, Candace K.

AU - Li, Wenguang

AU - Hicks, Raea K.

AU - Doorn, Stephen K.

AU - Wang, Hsing Lin

N1 - Funding Information: This work is supported by the LDRD program of Los Alamos National Laboratory, the Office of Science (DOE), and Cross Enterprise Technology Development Program from National Aeronautics and Space Administration, NASA. MJO acknowledges support from the DCI postdoctoral fellowship.

PY - 2007/12/13

Y1 - 2007/12/13

N2 - Non-conjugated polyelectrolytes were used to quench oppositely charged fluorescent dye-ligand conjugate (DLC) molecules by bringing them to the vicinity of the polyelectrolyte backbone to form aggregates and hence quench the dye fluorescence. As target protein molecules are added to the solution, the specific interaction between the DLC and the protein disrupts the aggregate structure, thus recovering the luminescence. The binding of DLC to oppositely charged polyelectrolyte and the disruption of the aggregates are investigated by fluorescence spectroscopy. The static-quenching mechanism is clearly manifested in the Stern-Volmer plots that show the decrease in slope with increasing temperature. This polyelectrolyte-based sensing platform has a sensitivity of <100 pM. We also show the selectivity of this platform by comparing the fluorescence recovery between two proteins (avidin and bovine serum albumin) with similar molecular weight. Our results suggest a highly sensitive approach for detecting biological analytes.

AB - Non-conjugated polyelectrolytes were used to quench oppositely charged fluorescent dye-ligand conjugate (DLC) molecules by bringing them to the vicinity of the polyelectrolyte backbone to form aggregates and hence quench the dye fluorescence. As target protein molecules are added to the solution, the specific interaction between the DLC and the protein disrupts the aggregate structure, thus recovering the luminescence. The binding of DLC to oppositely charged polyelectrolyte and the disruption of the aggregates are investigated by fluorescence spectroscopy. The static-quenching mechanism is clearly manifested in the Stern-Volmer plots that show the decrease in slope with increasing temperature. This polyelectrolyte-based sensing platform has a sensitivity of <100 pM. We also show the selectivity of this platform by comparing the fluorescence recovery between two proteins (avidin and bovine serum albumin) with similar molecular weight. Our results suggest a highly sensitive approach for detecting biological analytes.

KW - Electrostatic interaction

KW - Fluorescence

KW - Polyelectrolyte

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DO - 10.1016/j.polymer.2007.10.036

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JO - Polymer (United Kingdom)

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ER -

Polyelectrolyte platform for sensitive detection of biological analytes via reversible fluorescence quenching

Abstract

Keywords

ASJC Scopus subject areas

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