Poly(aminoether)-gold nanorod assemblies for shRNA plasmid-induced gene silencing

James Ramos, Kaushal Rege

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Gold nanorods (GNRs) have emerged as promising nanomaterials for biosensing, imaging, photothermal hyperthermia treatments, and therapeutic delivery for several diseases. We generated poly(aminoether)-GNR nanoassemblies using a layer-by-layer deposition approach based on the 1,4C-1,4Bis polymer from a library recently synthesized in our laboratory. Subtoxic concentrations of 1,4C-1,4Bis-GNR nanoassemblies were employed to deliver expression vectors that express shRNA ("shRNA plasmid") against firefly luciferase gene to knock down expression of the protein constitutively expressed in prostate cancer cells. The role of hydrodynamic size and zeta potential in determining nanoassembly mediated luciferase silencing was investigated. Finally, the theranostic potential of 1,4C-1,4Bis-GNR nanoassemblies was demonstrated using live cell two-photon induced luminescence bioimaging. Our results indicate that poly(aminoether)-GNR nanoassemblies are a promising theranostic platform for delivery of therapeutic payloads capable of simultaneous gene silencing and bioimaging.

Original languageEnglish (US)
Pages (from-to)4107-4119
Number of pages13
JournalMolecular Pharmaceutics
Volume10
Issue number11
DOIs
StatePublished - Nov 4 2013

Keywords

  • RNA interference
  • gold nanoparticles
  • nonviral gene silencing
  • shRNA delivery
  • shRNA plasmid
  • theranostics

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

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