Poliovirus induced inhibition of polypeptide initiation in vitro on native polyribosomes

Y. Kaufmann, E. Goldstein, S. Penman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The inhibition of HeLa cell protein synthesis by poliovirus was studied by examining initiation in vitro on endogenous host polyribosomes. At an early stage, before major viral RNA replication and protein synthesis begins, the initiation of translation on cellular mRNA is strongly inhibited. Fractionation of extracts from infected cells shows that the lesion is associated mainly with the crude polyribosome fraction. The cellular mRNA appears unchanged and is as active as mRNA from control cells in stimulating incorporation. The native ribosomal subunits and KCl washed polyribosomes from the infected cells are also active. Only the ribosomal wash fraction prepared from the inhibited polyribosomes has reduced activity. However, the reduction in the ribosomal wash activity measured in a reconstructed system is not as large as the inhibition seen with 'native' polyribosomes. The results indicate that a viral induced inhibition is probably associated with the ribosomal wash fraction, but the reconstructed system is not equivalent to the 'native' inhibited system.

Original languageEnglish (US)
Pages (from-to)1834-1838
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number6
StatePublished - 1976
Externally publishedYes

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Polyribosomes
Poliovirus
Peptides
Messenger RNA
Ribosome Subunits
Viral RNA
Viral Proteins
Cell Extracts
HeLa Cells
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Poliovirus induced inhibition of polypeptide initiation in vitro on native polyribosomes. / Kaufmann, Y.; Goldstein, E.; Penman, S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 73, No. 6, 1976, p. 1834-1838.

Research output: Contribution to journalArticle

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