Polarizability of Six-Helix Bundle and Triangle DNA Origami and Their Escape Characteristics from a Dielectrophoretic Trap

Lin Gan, Fernanda Camacho-Alanis, Alexandra Ros

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

DNA nanoassemblies, such as DNA origamis, hold promise in biosensing, drug delivery, nanoelectronic circuits, and biological computing, which require suitable methods for migration and precision positioning. Insulator-based dielectrophoresis (iDEP) has been demonstrated as a powerful migration and trapping tool for μm- and nm-sized colloids as well as DNA origamis. However, little is known about the polarizability of origami species, which is responsible for their dielectrophoretic migration. Here, we report the experimentally determined polarizabilities of the six-helix bundle origami (6HxB) and triangle origami by measuring the migration times through a potential landscape exhibiting dielectrophoretic barriers. The resulting migration times correlate to the depth of the dielectrophoretic potential barrier and the escape characteristics of the origami according to an adapted Kramer's rate model, allowing their polarizabilities to be determined. We found that the 6HxB polarizability is larger than that of the triangle origami, which correlates with the variations in charge density of both origamis. Further, we discuss the orientation of both origami species in the dielectrophoretic trap and discuss the influence of diffusion during the escape process. Our study provides detailed insight into the factors contributing to the migration through dielectrophoretic potential landscapes, which can be exploited for applications with DNA and other nanoassemblies based on dielectrophoresis.

Original languageEnglish (US)
Pages (from-to)12059-12064
Number of pages6
JournalAnalytical Chemistry
Volume87
Issue number24
DOIs
StatePublished - Dec 15 2015

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DNA
Electrophoresis
Nanoelectronics
Colloids
Charge density
Drug delivery
Networks (circuits)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Polarizability of Six-Helix Bundle and Triangle DNA Origami and Their Escape Characteristics from a Dielectrophoretic Trap. / Gan, Lin; Camacho-Alanis, Fernanda; Ros, Alexandra.

In: Analytical Chemistry, Vol. 87, No. 24, 15.12.2015, p. 12059-12064.

Research output: Contribution to journalArticle

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