Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy

Uwe Weierstall; John Spence; M. Stevens; K. H. Downing

doi:10.1016/S0968-4328(99)00022-0

Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy

Uwe Weierstall, John Spence, M. Stevens, K. H. Downing

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

In-line electron holograms of Tobacco mosaic virus (TMV) lying across holes in Quantifoil were recorded at 40 eV electron energy. The molecule is opaque at this energy and holographic reconstruction shows a projection image of the TMV. Radiation damage at this energy is expected to be confined to a thin surface layer of the molecule. Problems with the holographic reconstruction due to electric field inhomogeneity around the fiber are discussed.

Original language	English (US)
Pages (from-to)	335-338
Number of pages	4
Journal	Micron
Volume	30
Issue number	4
DOIs	https://doi.org/10.1016/S0968-4328(99)00022-0
State	Published - Aug 1999

Keywords

Electron holography
Point-projection microscopy
Radiation damage
Tobacco mosaic virus

ASJC Scopus subject areas

Structural Biology
Materials Science(all)
Physics and Astronomy(all)
Cell Biology

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10.1016/S0968-4328(99)00022-0

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title = "Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy",

abstract = "In-line electron holograms of Tobacco mosaic virus (TMV) lying across holes in Quantifoil were recorded at 40 eV electron energy. The molecule is opaque at this energy and holographic reconstruction shows a projection image of the TMV. Radiation damage at this energy is expected to be confined to a thin surface layer of the molecule. Problems with the holographic reconstruction due to electric field inhomogeneity around the fiber are discussed.",

keywords = "Electron holography, Point-projection microscopy, Radiation damage, Tobacco mosaic virus",

author = "Uwe Weierstall and John Spence and M. Stevens and Downing, {K. H.}",

year = "1999",

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doi = "10.1016/S0968-4328(99)00022-0",

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T1 - Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy

AU - Weierstall, Uwe

AU - Spence, John

AU - Stevens, M.

AU - Downing, K. H.

PY - 1999/8

Y1 - 1999/8

N2 - In-line electron holograms of Tobacco mosaic virus (TMV) lying across holes in Quantifoil were recorded at 40 eV electron energy. The molecule is opaque at this energy and holographic reconstruction shows a projection image of the TMV. Radiation damage at this energy is expected to be confined to a thin surface layer of the molecule. Problems with the holographic reconstruction due to electric field inhomogeneity around the fiber are discussed.

AB - In-line electron holograms of Tobacco mosaic virus (TMV) lying across holes in Quantifoil were recorded at 40 eV electron energy. The molecule is opaque at this energy and holographic reconstruction shows a projection image of the TMV. Radiation damage at this energy is expected to be confined to a thin surface layer of the molecule. Problems with the holographic reconstruction due to electric field inhomogeneity around the fiber are discussed.

KW - Electron holography

KW - Point-projection microscopy

KW - Radiation damage

KW - Tobacco mosaic virus

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U2 - 10.1016/S0968-4328(99)00022-0

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JO - Zeitschrift fur wissenschaftliche Mikroskopie und mikroskopische Technik

JF - Zeitschrift fur wissenschaftliche Mikroskopie und mikroskopische Technik

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ER -

Point-projection electron imaging of tobacco mosaic virus at 40 eV electron energy

Abstract

Keywords

ASJC Scopus subject areas

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