Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer

Ran Zou, Wenbo Cao, Leelyn Chong, Wei Hua, Hao Xu, Ying Mao, Jessica Page, Riyi Shi, Yu Xia, Ye Hu, Wenpeng Zhang, Zheng Ouyang

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paternò-Büchi) reaction, fast identification of lipid C=C location was realized. Relative quantitation of the lipid C=C isomer was performed by calculating the intensity ratio C=C diagnostic product ions, by which FA 18:1 (Δ9)/FA 18:1 (Δ11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.

Original languageEnglish (US)
Pages (from-to)1157-1163
Number of pages7
JournalAnalytical chemistry
Volume91
Issue number1
DOIs
StatePublished - Jan 2 2019

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Zou, R., Cao, W., Chong, L., Hua, W., Xu, H., Mao, Y., Page, J., Shi, R., Xia, Y., Hu, Y., Zhang, W., & Ouyang, Z. (2019). Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer. Analytical chemistry, 91(1), 1157-1163. https://doi.org/10.1021/acs.analchem.8b04935