TY - JOUR
T1 - Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer
AU - Zou, Ran
AU - Cao, Wenbo
AU - Chong, Leelyn
AU - Hua, Wei
AU - Xu, Hao
AU - Mao, Ying
AU - Page, Jessica
AU - Shi, Riyi
AU - Xia, Yu
AU - Hu, Ye
AU - Zhang, Wenpeng
AU - Ouyang, Zheng
N1 - Funding Information:
Financial support from National Natural Science Foundation of China (Project 21627807) and National Institutes of Health (Project R44GM119584, 1R01AI122298 and R01AI122932) is greatly appreciated.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2019/1/2
Y1 - 2019/1/2
N2 - The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paternò-Büchi) reaction, fast identification of lipid C=C location was realized. Relative quantitation of the lipid C=C isomer was performed by calculating the intensity ratio C=C diagnostic product ions, by which FA 18:1 (Δ9)/FA 18:1 (Δ11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.
AB - The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paternò-Büchi) reaction, fast identification of lipid C=C location was realized. Relative quantitation of the lipid C=C isomer was performed by calculating the intensity ratio C=C diagnostic product ions, by which FA 18:1 (Δ9)/FA 18:1 (Δ11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.
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U2 - 10.1021/acs.analchem.8b04935
DO - 10.1021/acs.analchem.8b04935
M3 - Article
C2 - 30525456
AN - SCOPUS:85059607987
SN - 0003-2700
VL - 91
SP - 1157
EP - 1163
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 1
ER -