PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering

Ali Navaei, Danh Truong, John Heffernan, Josh Cutts, David Brafman, Rachael W. Sirianni, Brent Vernon, Mehdi Nikkhah

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Injectable biomaterials offer a non-invasive approach to deliver cells into the myocardial infarct region to maintain a high level of cell retention and viability and initiate the regeneration process. However, previously developed injectable matrices often suffer from low bioactivity or poor mechanical properties. To address this need, we introduced a biohybrid temperature-responsive poly(N-isopropylacrylamide) PNIPAAm-Gelatin-based injectable hydrogel with excellent bioactivity as well as mechanical robustness for cardiac tissue engineering. A unique feature of our work was that we performed extensive in vitro biological analyses to assess the functionalities of cardiomyocytes (CMs) alone and in co-culture with cardiac fibroblasts (CFS) (2:1 ratio) within the hydrogel matrix. The synthesized hydrogel exhibited viscoelastic behavior (storage modulus: 1260 Pa) and necessary water content (75%) to properly accommodate the cardiac cells. The encapsulated cells demonstrated a high level of cell survival (90% for co-culture condition, day 7) and spreading throughout the hydrogel matrix in both culture conditions. A dense network of stained F-actin fibers (∼6 × 104 μm2 area coverage, co-culture condition) illustrated the formation of an intact and three dimensional (3D) cell-embedded matrix. Furthermore, immunostaining and gene expression analyses revealed mature phenotypic characteristics of cardiac cells. Notably, the co-culture group exhibited superior structural organization and cell-cell coupling, as well as beating behavior (average ∼45 beats per min, co-culture condition, day 7). The outcome of this study is envisioned to open a new avenue for extensive in vitro characterization of injectable matrices embedded with 3D mono- and co-culture of cardiac cells prior to in vivo experiments. Statement of Significance In this work, we synthesized a new class of biohybrid temperature-responsive poly(N-isopropylacrylamide) PNIPAAm-Gelatin-based injectable hydrogel with suitable bioactivity and mechanical properties for cardiac tissue engineering. A significant aspect of our work was that we performed extensive in vitro biological analyses to assess the functionality of cardiomyocytes alone and in co-culture with cardiac fibroblasts encapsulated within the 3D hydrogel matrix.

Original languageEnglish (US)
Pages (from-to)10-23
Number of pages14
JournalActa Biomaterialia
Volume32
DOIs
StatePublished - Mar 1 2016

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Hydrogel
Tissue Engineering
Tissue engineering
Hydrogels
Coculture Techniques
Injections
Bioactivity
Cell culture
Fibroblasts
Gelatin
Cardiac Myocytes
Mechanical properties
Cell Survival
Biocompatible Materials
Gene expression
Biomaterials
Water content
Temperature
poly-N-isopropylacrylamide
Actins

Keywords

  • Cardiac fibroblast
  • Cell encapsulation
  • Co-culture
  • Contraction behavior
  • Gelatin
  • Gene expression
  • PNIPAAm

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering. / Navaei, Ali; Truong, Danh; Heffernan, John; Cutts, Josh; Brafman, David; Sirianni, Rachael W.; Vernon, Brent; Nikkhah, Mehdi.

In: Acta Biomaterialia, Vol. 32, 01.03.2016, p. 10-23.

Research output: Contribution to journalArticle

Navaei, Ali ; Truong, Danh ; Heffernan, John ; Cutts, Josh ; Brafman, David ; Sirianni, Rachael W. ; Vernon, Brent ; Nikkhah, Mehdi. / PNIPAAm-based biohybrid injectable hydrogel for cardiac tissue engineering. In: Acta Biomaterialia. 2016 ; Vol. 32. pp. 10-23.
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AU - Vernon, Brent

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