Plastome Structural Evolution and Homoplastic Inversions in Neo-Astragalus (Fabaceae)

Joseph L.M. Charboneau, Richard C. Cronn, Aaron Liston, Martin F. Wojciechowski, Michael J. Sanderson

Research output: Contribution to journalArticlepeer-review

Abstract

The plastid genomes of photosynthetic green plants have largely maintained conserved gene content and order as well as structure over hundreds of millions of years of evolution. Several plant lineages, however, have departed from this conservation and contain many plastome structural rearrangements, which have been associated with an abundance of repeated sequences both overall and near rearrangement endpoints. We sequenced the plastomes of 25 taxa of Astragalus L. (Fabaceae), a large genus in the inverted repeat-lacking clade of legumes, to gain a greater understanding of the connection between repeats and plastome inversions. We found plastome repeat structure has a strong phylogenetic signal among these closely related taxa mostly in the New World clade of Astragalus called Neo-Astragalus. Taxa without inversions also do not differ substantially in their overall repeat structure from four taxa each with one large-scale inversion. For two taxa with inversion endpoints between the same pairs of genes, differences in their exact endpoints indicate the inversions occurred independently. Our proposed mechanism for inversion formation suggests the short inverted repeats now found near the endpoints of the four inversions may be there as a result of these inversions rather than their cause. The longer inverted repeats now near endpoints may have allowed the inversions first mediated by shorter microhomologous sequences to propagate, something that should be considered in explaining how any plastome rearrangement becomes fixed regardless of the mechanism of initial formation.

Original languageEnglish (US)
JournalGenome biology and evolution
Volume13
Issue number10
DOIs
StatePublished - Oct 1 2021

Keywords

  • chloroplast
  • inverted repeat-lacking clade
  • legumes
  • microhomology-mediated rearrangements
  • plastid genome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Fingerprint

Dive into the research topics of 'Plastome Structural Evolution and Homoplastic Inversions in Neo-Astragalus (Fabaceae)'. Together they form a unique fingerprint.

Cite this