Abstract
Plasmonic-based electrochemical impedance spectroscopy (P-EIS) is developed to investigate molecular binding on surfaces. Its basic principle relies on the sensitive dependence of surface plasmon resonance (SPR) signal on surface charge density, which is modulated by applying an ac potential to a SPR chip surface. The ac component of the SPR response gives the electrochemical impedance, and the dc component provides the conventional SPR detection. The plasmonic-based impedance measured over a range of frequency is in quantitative agreement with the conventional electrochemical impedance. Compared to the conventional SPR detection, P-EIS is sensitive to molecular binding taking place on the chip surface and less sensitive to bulk refractive index changes or nonspecific binding. Moreover, this new approach allows for simultaneous SPR and surface impedance analysis of molecular binding processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-333 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 84 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 3 2012 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Plasmonic-based electrochemical impedance spectroscopy : Application to molecular binding. / Lu, Jin; Wang, Wei; Wang, Shaopeng; Shan, Xiaonan; Li, Jinghong; Tao, Nongjian.
In: Analytical Chemistry, Vol. 84, No. 1, 03.01.2012, p. 327-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasmonic-based electrochemical impedance spectroscopy
T2 - Application to molecular binding
AU - Lu, Jin
AU - Wang, Wei
AU - Wang, Shaopeng
AU - Shan, Xiaonan
AU - Li, Jinghong
AU - Tao, Nongjian
PY - 2012/1/3
Y1 - 2012/1/3
N2 - Plasmonic-based electrochemical impedance spectroscopy (P-EIS) is developed to investigate molecular binding on surfaces. Its basic principle relies on the sensitive dependence of surface plasmon resonance (SPR) signal on surface charge density, which is modulated by applying an ac potential to a SPR chip surface. The ac component of the SPR response gives the electrochemical impedance, and the dc component provides the conventional SPR detection. The plasmonic-based impedance measured over a range of frequency is in quantitative agreement with the conventional electrochemical impedance. Compared to the conventional SPR detection, P-EIS is sensitive to molecular binding taking place on the chip surface and less sensitive to bulk refractive index changes or nonspecific binding. Moreover, this new approach allows for simultaneous SPR and surface impedance analysis of molecular binding processes.
AB - Plasmonic-based electrochemical impedance spectroscopy (P-EIS) is developed to investigate molecular binding on surfaces. Its basic principle relies on the sensitive dependence of surface plasmon resonance (SPR) signal on surface charge density, which is modulated by applying an ac potential to a SPR chip surface. The ac component of the SPR response gives the electrochemical impedance, and the dc component provides the conventional SPR detection. The plasmonic-based impedance measured over a range of frequency is in quantitative agreement with the conventional electrochemical impedance. Compared to the conventional SPR detection, P-EIS is sensitive to molecular binding taking place on the chip surface and less sensitive to bulk refractive index changes or nonspecific binding. Moreover, this new approach allows for simultaneous SPR and surface impedance analysis of molecular binding processes.
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UR - http://www.scopus.com/inward/citedby.url?scp=84855397425&partnerID=8YFLogxK
U2 - 10.1021/ac202634h
DO - 10.1021/ac202634h
M3 - Article
C2 - 22122514
AN - SCOPUS:84855397425
VL - 84
SP - 327
EP - 333
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 1
ER -