TY - JOUR
T1 - Plasma-treated polystyrene film that enhances binding efficiency for sensitive and label-free protein biosensing
AU - Guo, Bihong
AU - Li, Shaopeng
AU - Song, Lusheng
AU - Yang, Mo
AU - Zhou, Wenfei
AU - Tyagi, Deependra
AU - Zhu, Jinsong
N1 - Funding Information:
This research was supported by National Major Scientific Instruments and Equipment Development Project ( 2011YQ03012405 ), National Natural Science Foundation of China ( 61077064/60921001 ) and Program of International S&T Cooperation ( 2010DFB33880 ).
Publisher Copyright:
© 2015 Published by Elsevier B.V.
PY - 2015
Y1 - 2015
N2 - A plasma-treated ultrathin polystyrene (PS) film surface was explored as a simple, robust, and low-cost surface chemistry solution for protein biosensing applications. This surface could dramatically improve the binding efficiency of the protein-protein interactions, which is defined as the binding signal per immobilized ligand. The PS-modified protein biosensor was readily fabricated by spin coating and plasma treatment. Various parameters for fabrication, including the concentration of the PS solution, rate of spin coating, and duration of plasma treatment, were systematically optimized based on the improvement of fluorescence signal yielded by the microfluidic network-aided fluorescence immunoassay. The performance of the label-free protein detection on the optimized surfaces was further evaluated by surface plasmon resonance imaging (SPRi). PS surfaces with optimal fabrication parameters exhibited up to an 620% enhancement of the protein binding response and approximately 210% of the protein binding per immobilized protein ligand compared with a self-assembled monolayer (SAM) surface of 11-mercapto undecanoic acid (MUA). The relationship between the fabrication parameters used and changes to the surface chemistry and the morphological properties were characterized with atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). It was revealed that the morphological changes observed in the plasma-treated PS film were the dominant factor for the improvement of the protein bioassay performance, rather than the chemical changes.
AB - A plasma-treated ultrathin polystyrene (PS) film surface was explored as a simple, robust, and low-cost surface chemistry solution for protein biosensing applications. This surface could dramatically improve the binding efficiency of the protein-protein interactions, which is defined as the binding signal per immobilized ligand. The PS-modified protein biosensor was readily fabricated by spin coating and plasma treatment. Various parameters for fabrication, including the concentration of the PS solution, rate of spin coating, and duration of plasma treatment, were systematically optimized based on the improvement of fluorescence signal yielded by the microfluidic network-aided fluorescence immunoassay. The performance of the label-free protein detection on the optimized surfaces was further evaluated by surface plasmon resonance imaging (SPRi). PS surfaces with optimal fabrication parameters exhibited up to an 620% enhancement of the protein binding response and approximately 210% of the protein binding per immobilized protein ligand compared with a self-assembled monolayer (SAM) surface of 11-mercapto undecanoic acid (MUA). The relationship between the fabrication parameters used and changes to the surface chemistry and the morphological properties were characterized with atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). It was revealed that the morphological changes observed in the plasma-treated PS film were the dominant factor for the improvement of the protein bioassay performance, rather than the chemical changes.
KW - Plasma treatment
KW - Polystyrene film
KW - Protein binding efficiency
KW - Protein biosensor
KW - Surface plasmon resonance imaging
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U2 - 10.1016/j.apsusc.2015.03.070
DO - 10.1016/j.apsusc.2015.03.070
M3 - Article
AN - SCOPUS:84933564061
SN - 0169-4332
VL - 345
SP - 379
EP - 386
JO - Applied Surface Science
JF - Applied Surface Science
ER -