Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets

Fayth L. Miles, Sandi L. Navarro, Yvonne Schwarz, Haiwei Gu, Danijel Djukovic, Timothy W. Randolph, Ali Shojaie, Mario Kratz, Meredith A.J. Hullar, Paul D. Lampe, Marian L. Neuhouser, Daniel Raftery, Johanna W. Lampe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Enterolignans, products of gut bacterial metabolism of plant lignans, have been associated with reduced risk of chronic diseases, but their association with other plasma metabolites is unknown. We examined plasma metabolite profiles according to urinary enterolignan excretion in a cross-sectional analysis using data from a randomized crossover, controlled feeding study. Eighty healthy adult males and females completed two 28-day feeding periods differing by glycemic load, refined carbohydrate, and fiber content. Lignan intake was calculated from food records using a polyphenol database. Targeted metabolomics was performed by LC-MS on plasma from fasting blood samples collected at the end of each feeding period. Enterolactone (ENL) and enterodiol, were measured in 24 h urine samples collected on the penultimate day of each study period using GC-MS. Linear mixed models were used to test the association between enterolignan excretion and metabolite abundances. Pathway analyses were conducted using the Global Test. Benjamini-Hochberg false discovery rate (FDR) was used to control for multiple testing. Of the metabolites assayed, 121 were detected in all samples. ENL excretion was associated positively with plasma hippuric acid and melatonin, and inversely with epinephrine, creatine, glycochenodeoxycholate, and glyceraldehyde (P < 0.05). Hippuric acid only satisfied the FDR of q < 0.1. END excretion was associated with myristic acid and glycine (q < 0.5). Two of 57 pathways tested were associated significantly with ENL, ubiquinone and terpenoid-quinone biosynthesis, and inositol phosphate metabolism. These results suggest a potential role for ENL or ENL-metabolizing gut bacteria in regulating plasma metabolites.

Original languageEnglish (US)
Pages (from-to)3209-3218
Number of pages10
JournalFood and Function
Volume8
Issue number9
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

Fingerprint

Healthy Volunteers
excretion
metabolites
Diet
hippuric acid
Lignans
diet
lignans
Glycochenodeoxycholic Acid
digestive system
glyceraldehyde
Glyceraldehyde
inositol phosphates
creatine
myristic acid
Ubiquinone
metabolism
Inositol Phosphates
Metabolomics
Creatine

ASJC Scopus subject areas

  • Food Science

Cite this

Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets. / Miles, Fayth L.; Navarro, Sandi L.; Schwarz, Yvonne; Gu, Haiwei; Djukovic, Danijel; Randolph, Timothy W.; Shojaie, Ali; Kratz, Mario; Hullar, Meredith A.J.; Lampe, Paul D.; Neuhouser, Marian L.; Raftery, Daniel; Lampe, Johanna W.

In: Food and Function, Vol. 8, No. 9, 01.09.2017, p. 3209-3218.

Research output: Contribution to journalArticle

Miles, FL, Navarro, SL, Schwarz, Y, Gu, H, Djukovic, D, Randolph, TW, Shojaie, A, Kratz, M, Hullar, MAJ, Lampe, PD, Neuhouser, ML, Raftery, D & Lampe, JW 2017, 'Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets', Food and Function, vol. 8, no. 9, pp. 3209-3218. https://doi.org/10.1039/c7fo00684e
Miles, Fayth L. ; Navarro, Sandi L. ; Schwarz, Yvonne ; Gu, Haiwei ; Djukovic, Danijel ; Randolph, Timothy W. ; Shojaie, Ali ; Kratz, Mario ; Hullar, Meredith A.J. ; Lampe, Paul D. ; Neuhouser, Marian L. ; Raftery, Daniel ; Lampe, Johanna W. / Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets. In: Food and Function. 2017 ; Vol. 8, No. 9. pp. 3209-3218.
@article{9dd540c4237642c29224e80749d030b8,
title = "Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets",
abstract = "Enterolignans, products of gut bacterial metabolism of plant lignans, have been associated with reduced risk of chronic diseases, but their association with other plasma metabolites is unknown. We examined plasma metabolite profiles according to urinary enterolignan excretion in a cross-sectional analysis using data from a randomized crossover, controlled feeding study. Eighty healthy adult males and females completed two 28-day feeding periods differing by glycemic load, refined carbohydrate, and fiber content. Lignan intake was calculated from food records using a polyphenol database. Targeted metabolomics was performed by LC-MS on plasma from fasting blood samples collected at the end of each feeding period. Enterolactone (ENL) and enterodiol, were measured in 24 h urine samples collected on the penultimate day of each study period using GC-MS. Linear mixed models were used to test the association between enterolignan excretion and metabolite abundances. Pathway analyses were conducted using the Global Test. Benjamini-Hochberg false discovery rate (FDR) was used to control for multiple testing. Of the metabolites assayed, 121 were detected in all samples. ENL excretion was associated positively with plasma hippuric acid and melatonin, and inversely with epinephrine, creatine, glycochenodeoxycholate, and glyceraldehyde (P < 0.05). Hippuric acid only satisfied the FDR of q < 0.1. END excretion was associated with myristic acid and glycine (q < 0.5). Two of 57 pathways tested were associated significantly with ENL, ubiquinone and terpenoid-quinone biosynthesis, and inositol phosphate metabolism. These results suggest a potential role for ENL or ENL-metabolizing gut bacteria in regulating plasma metabolites.",
author = "Miles, {Fayth L.} and Navarro, {Sandi L.} and Yvonne Schwarz and Haiwei Gu and Danijel Djukovic and Randolph, {Timothy W.} and Ali Shojaie and Mario Kratz and Hullar, {Meredith A.J.} and Lampe, {Paul D.} and Neuhouser, {Marian L.} and Daniel Raftery and Lampe, {Johanna W.}",
year = "2017",
month = "9",
day = "1",
doi = "10.1039/c7fo00684e",
language = "English (US)",
volume = "8",
pages = "3209--3218",
journal = "Food and Function",
issn = "2042-6496",
publisher = "Royal Society of Chemistry",
number = "9",

}

TY - JOUR

T1 - Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets

AU - Miles, Fayth L.

AU - Navarro, Sandi L.

AU - Schwarz, Yvonne

AU - Gu, Haiwei

AU - Djukovic, Danijel

AU - Randolph, Timothy W.

AU - Shojaie, Ali

AU - Kratz, Mario

AU - Hullar, Meredith A.J.

AU - Lampe, Paul D.

AU - Neuhouser, Marian L.

AU - Raftery, Daniel

AU - Lampe, Johanna W.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Enterolignans, products of gut bacterial metabolism of plant lignans, have been associated with reduced risk of chronic diseases, but their association with other plasma metabolites is unknown. We examined plasma metabolite profiles according to urinary enterolignan excretion in a cross-sectional analysis using data from a randomized crossover, controlled feeding study. Eighty healthy adult males and females completed two 28-day feeding periods differing by glycemic load, refined carbohydrate, and fiber content. Lignan intake was calculated from food records using a polyphenol database. Targeted metabolomics was performed by LC-MS on plasma from fasting blood samples collected at the end of each feeding period. Enterolactone (ENL) and enterodiol, were measured in 24 h urine samples collected on the penultimate day of each study period using GC-MS. Linear mixed models were used to test the association between enterolignan excretion and metabolite abundances. Pathway analyses were conducted using the Global Test. Benjamini-Hochberg false discovery rate (FDR) was used to control for multiple testing. Of the metabolites assayed, 121 were detected in all samples. ENL excretion was associated positively with plasma hippuric acid and melatonin, and inversely with epinephrine, creatine, glycochenodeoxycholate, and glyceraldehyde (P < 0.05). Hippuric acid only satisfied the FDR of q < 0.1. END excretion was associated with myristic acid and glycine (q < 0.5). Two of 57 pathways tested were associated significantly with ENL, ubiquinone and terpenoid-quinone biosynthesis, and inositol phosphate metabolism. These results suggest a potential role for ENL or ENL-metabolizing gut bacteria in regulating plasma metabolites.

AB - Enterolignans, products of gut bacterial metabolism of plant lignans, have been associated with reduced risk of chronic diseases, but their association with other plasma metabolites is unknown. We examined plasma metabolite profiles according to urinary enterolignan excretion in a cross-sectional analysis using data from a randomized crossover, controlled feeding study. Eighty healthy adult males and females completed two 28-day feeding periods differing by glycemic load, refined carbohydrate, and fiber content. Lignan intake was calculated from food records using a polyphenol database. Targeted metabolomics was performed by LC-MS on plasma from fasting blood samples collected at the end of each feeding period. Enterolactone (ENL) and enterodiol, were measured in 24 h urine samples collected on the penultimate day of each study period using GC-MS. Linear mixed models were used to test the association between enterolignan excretion and metabolite abundances. Pathway analyses were conducted using the Global Test. Benjamini-Hochberg false discovery rate (FDR) was used to control for multiple testing. Of the metabolites assayed, 121 were detected in all samples. ENL excretion was associated positively with plasma hippuric acid and melatonin, and inversely with epinephrine, creatine, glycochenodeoxycholate, and glyceraldehyde (P < 0.05). Hippuric acid only satisfied the FDR of q < 0.1. END excretion was associated with myristic acid and glycine (q < 0.5). Two of 57 pathways tested were associated significantly with ENL, ubiquinone and terpenoid-quinone biosynthesis, and inositol phosphate metabolism. These results suggest a potential role for ENL or ENL-metabolizing gut bacteria in regulating plasma metabolites.

UR - http://www.scopus.com/inward/record.url?scp=85029750578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029750578&partnerID=8YFLogxK

U2 - 10.1039/c7fo00684e

DO - 10.1039/c7fo00684e

M3 - Article

VL - 8

SP - 3209

EP - 3218

JO - Food and Function

JF - Food and Function

SN - 2042-6496

IS - 9

ER -