Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana

Julio Paez-Valencia, Araceli Patron-Soberano, Alejandra Rodriguez-Leviz, Jonathan Sanchez-Lares, Concepcion Sanchez-Gomez, Pedro Valencia-Mayoral, Guadalupe Diaz-Rosas, Roberto Gaxiola

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Previous literature has shown the presence of a plasma membrane (PM) localized type I H+-PPase in sieve elements of Ricinus communis. Unfortunately, the physiological relevance of these findings remains obscure due to the lack of genetic and molecular reagents to study R. communis. The availability of H+-PPase gain and loss-of-function mutants in Arabidopsis thaliana makes this plant an attractive genetic model to address the question, but data on the PM localization of this H+-PPase in A. thaliana are limited to two proteomic approaches. Here we present the first report on the localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes (SE-CCc) from A. thaliana. Double epifluorescence and immunogold labeling experiments are consistent with the co-localization of AVP1 and PIP1 (a bona fide PM maker) in PM of SE-CCc from A. thaliana.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalPlant Science
Volume181
Issue number1
DOIs
StatePublished - Jul 2011

Fingerprint

Inorganic Pyrophosphatase
phloem companion cells
sieve elements
Arabidopsis
plasma membrane
Arabidopsis thaliana
Cell Membrane
Ricinus
Ricinus communis
plant genetics
Genetic Models
Proteomics
proteomics
Molecular Biology
mutants

Keywords

  • Arabidopsis thaliana
  • H-PPase
  • Phloem
  • Sieves elements-companion cell complex
  • Sucrose

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

Paez-Valencia, J., Patron-Soberano, A., Rodriguez-Leviz, A., Sanchez-Lares, J., Sanchez-Gomez, C., Valencia-Mayoral, P., ... Gaxiola, R. (2011). Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana. Plant Science, 181(1), 23-30. https://doi.org/10.1016/j.plantsci.2011.03.008

Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana. / Paez-Valencia, Julio; Patron-Soberano, Araceli; Rodriguez-Leviz, Alejandra; Sanchez-Lares, Jonathan; Sanchez-Gomez, Concepcion; Valencia-Mayoral, Pedro; Diaz-Rosas, Guadalupe; Gaxiola, Roberto.

In: Plant Science, Vol. 181, No. 1, 07.2011, p. 23-30.

Research output: Contribution to journalArticle

Paez-Valencia, J, Patron-Soberano, A, Rodriguez-Leviz, A, Sanchez-Lares, J, Sanchez-Gomez, C, Valencia-Mayoral, P, Diaz-Rosas, G & Gaxiola, R 2011, 'Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana', Plant Science, vol. 181, no. 1, pp. 23-30. https://doi.org/10.1016/j.plantsci.2011.03.008
Paez-Valencia J, Patron-Soberano A, Rodriguez-Leviz A, Sanchez-Lares J, Sanchez-Gomez C, Valencia-Mayoral P et al. Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana. Plant Science. 2011 Jul;181(1):23-30. https://doi.org/10.1016/j.plantsci.2011.03.008
Paez-Valencia, Julio ; Patron-Soberano, Araceli ; Rodriguez-Leviz, Alejandra ; Sanchez-Lares, Jonathan ; Sanchez-Gomez, Concepcion ; Valencia-Mayoral, Pedro ; Diaz-Rosas, Guadalupe ; Gaxiola, Roberto. / Plasma membrane localization of the type I H+-PPase AVP1 in sieve element-companion cell complexes from Arabidopsis thaliana. In: Plant Science. 2011 ; Vol. 181, No. 1. pp. 23-30.
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