Plant proton pumping pyrophosphatase

the potential for its pyrophosphate synthesis activity to modulate plant growth

C. Primo, G. A. Pizzio, J. Yang, Roberto Gaxiola, J. Scholz-Starke, K. D. Hirschi

Research output: Contribution to journalReview article

Abstract

Cellular pyrophosphate (PPi) homeostasis is vital for normal plant growth and development. Plant proton-pumping pyrophosphatases (H+-PPases) are enzymes with different tissue-specific functions related to the regulation of PPi homeostasis. Enhanced expression of plant H+-PPases increases biomass and yield in different crop species. Here, we emphasise emerging studies utilising heterologous expression in yeast and plant vacuole electrophysiology approaches, as well as phylogenetic relationships and structural analysis, to showcase that the H+-PPases possess a PPi synthesis function. We postulate this synthase activity contributes to modulating and promoting plant growth both in H+-PPase-engineered crops and in wild-type plants. We propose a model where the PPi synthase activity of H+-PPases maintains the PPi pool when cells adopt PPi-dependent glycolysis during high energy demands and/or low oxygen environments. We conclude by proposing experiments to further investigate the H+-PPase-mediated PPi synthase role in plant growth.

Original languageEnglish (US)
JournalPlant Biology
DOIs
StatePublished - Jan 1 2019

Fingerprint

pyrophosphatases
pyrophosphates
protons
pumping
plant growth
synthesis
homeostasis
electrophysiology
glycolysis
vacuoles
plant development
crop yield
growth and development
crop
yeasts
oxygen
structural analysis
biomass
phylogeny
energy

Keywords

  • Arabidopsis thaliana
  • Arabidopsis Vacuolar proton-pumping Pyrophosphatase type I
  • H-pyrophosphatase
  • pyrophosphate
  • pyrophosphate synthase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Plant proton pumping pyrophosphatase : the potential for its pyrophosphate synthesis activity to modulate plant growth. / Primo, C.; Pizzio, G. A.; Yang, J.; Gaxiola, Roberto; Scholz-Starke, J.; Hirschi, K. D.

In: Plant Biology, 01.01.2019.

Research output: Contribution to journalReview article

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AU - Primo, C.

AU - Pizzio, G. A.

AU - Yang, J.

AU - Gaxiola, Roberto

AU - Scholz-Starke, J.

AU - Hirschi, K. D.

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N2 - Cellular pyrophosphate (PPi) homeostasis is vital for normal plant growth and development. Plant proton-pumping pyrophosphatases (H+-PPases) are enzymes with different tissue-specific functions related to the regulation of PPi homeostasis. Enhanced expression of plant H+-PPases increases biomass and yield in different crop species. Here, we emphasise emerging studies utilising heterologous expression in yeast and plant vacuole electrophysiology approaches, as well as phylogenetic relationships and structural analysis, to showcase that the H+-PPases possess a PPi synthesis function. We postulate this synthase activity contributes to modulating and promoting plant growth both in H+-PPase-engineered crops and in wild-type plants. We propose a model where the PPi synthase activity of H+-PPases maintains the PPi pool when cells adopt PPi-dependent glycolysis during high energy demands and/or low oxygen environments. We conclude by proposing experiments to further investigate the H+-PPase-mediated PPi synthase role in plant growth.

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