Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis

Vinay J. Nagaraj, Seron Eaton, Derek Thirstrup, Peter Wiktor

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Glycans have great potential as disease biomarkers and therapeutic targets. However, the major challenge for glycan biomarker identification from clinical samples is the low abundance of key glycosylated proteins. To demonstrate the potential for glycan analysis with nanoliter amounts of glycoprotein, we have developed a new technology (Lectin NanoProbeArray) based on piezoelectric liquid dispensing for non-contact printing and probing of a lectin array. Instead of flooding the glycoprotein probe on the lectin array surface, as in conventional microarray screening, a piezoelectric printer is used to dispense nanoliters of fluorescently labeled glycoprotein probe over the lectin spots on the array. As a proof-of-concept, the ability of Lectin NanoProbeArrays to precisely identify and reliably distinguish between the closely related glycoforms of fetuin is illustrated here. Sensitivity levels comparable to lectin arrays that use evanescent-field scanners was achieved along with several orders of magnitude reduction in the amount of probe required for glycosylation analysis.

Original languageEnglish (US)
Pages (from-to)526-530
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume375
Issue number4
DOIs
StatePublished - Oct 31 2008

Keywords

  • Glycoprofiling
  • Glycosylation analysis
  • Lectin Microarray
  • Lectin NanoProbeArray
  • Piezoelectric liquid dispensing

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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