Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria

Bruch K. Mobarry, Michael Wagner, Vincent Urbain, Bruce Rittmann, David A. Stahl

Research output: Contribution to journalArticle

743 Citations (Scopus)

Abstract

A hierarchical set of five 16S rRNA-targeted oligonucleotide DNA probes for phylogenetically defined groups of autotrophic ammonia- and nitrite- oxidizing bacteria was developed for environmental and determinative studies. Hybridization conditions were established for each probe by using temperature dissociation profiles of target and closely related nontarget organisms to document specificity. Environmental application was demonstrated by quantitative slot blot hybridization and whole-cell hybridization of nitrifying activated sludge and biofilm samples. Results obtained with both techniques suggested the occurrence of novel populations of ammonia oxidizers. In situ hybridization experiments revealed that Nitrobacter and Nitrosomonas species occurred in clusters and frequently were in contact with each other within sludge flocs.

Original languageEnglish (US)
Pages (from-to)2156-2162
Number of pages7
JournalApplied and Environmental Microbiology
Volume62
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

nitrifying bacterium
Sewage
Ammonia
Nitrobacter
Nitrosomonas
hybridization
probe
Bacteria
phylogenetics
Oligonucleotide Probes
phylogeny
DNA Probes
ammonia
Biofilms
Nitrites
In Situ Hybridization
activated sludge
nontarget organisms
DNA probes
oligonucleotides

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. / Mobarry, Bruch K.; Wagner, Michael; Urbain, Vincent; Rittmann, Bruce; Stahl, David A.

In: Applied and Environmental Microbiology, Vol. 62, No. 6, 1996, p. 2156-2162.

Research output: Contribution to journalArticle

Mobarry, Bruch K. ; Wagner, Michael ; Urbain, Vincent ; Rittmann, Bruce ; Stahl, David A. / Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. In: Applied and Environmental Microbiology. 1996 ; Vol. 62, No. 6. pp. 2156-2162.
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