Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase

Lukasz Kedzierski, Ananias A. Escalante, Raul Isea, Casilda G. Black, John W. Barnwell, Ross L. Coppel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phylogenetic studies of the genus Plasmodium have been performed using sequences of the nuclear, mitochondrial and plastid genes. Here we have analyzed the adenylosuccinate lyase (ASL) gene, which encodes an enzyme involved in the salvage of host purines needed by malaria parasites for DNA synthesis. The ASL gene is present in several eukaryotic as well as prokaryotic organisms and does not have repeat regions, which facilitates the accuracy of the alignment. Furthermore, it has been shown that ASL is not subject to positive natural selection. We have sequenced the ASL gene of several different Plasmodium species infecting humans, rodents, monkeys and birds and used the obtained sequences along with the previously known P. falciparum ASL sequence, for structural and phylogenetic analysis of the genus Plasmodium. The genetic divergence of ASL is comparable with that observed in other nuclear genes such as cysteine proteinase, although ASL cannot be considered conserved when compared to aldolase or superoxide dismutase, which exhibit a slower rate of evolution. Nevertheless, a protein like ASL has a rate of evolution that provides enough information for elucidating evolutionary relationships. We modeled 3D structures of the ASL protein based on sequences used in the phylogenetic analysis and obtained a consistent structure for four different species despite the divergence observed. Such models would facilitate alignment in further studies with a greater number of plasmodial species or other Apicomplexa.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalInfection, Genetics and Evolution
Volume1
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Adenylosuccinate Lyase
Plasmodium
lyases
phylogenetics
gene
phylogeny
Genes
genes
divergence
protein
malaria
plastid
natural selection
rodent
parasite
Apicomplexa
analysis
fructose-bisphosphate aldolase
enzyme
Purines

Keywords

  • Adenylosuccinate lyase
  • Malaria
  • Phylogenetic analysis
  • Plasmodium

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

Cite this

Kedzierski, L., Escalante, A. A., Isea, R., Black, C. G., Barnwell, J. W., & Coppel, R. L. (2002). Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase. Infection, Genetics and Evolution, 1(4), 297-301. https://doi.org/10.1016/S1567-1348(02)00031-X

Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase. / Kedzierski, Lukasz; Escalante, Ananias A.; Isea, Raul; Black, Casilda G.; Barnwell, John W.; Coppel, Ross L.

In: Infection, Genetics and Evolution, Vol. 1, No. 4, 2002, p. 297-301.

Research output: Contribution to journalArticle

Kedzierski, L, Escalante, AA, Isea, R, Black, CG, Barnwell, JW & Coppel, RL 2002, 'Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase', Infection, Genetics and Evolution, vol. 1, no. 4, pp. 297-301. https://doi.org/10.1016/S1567-1348(02)00031-X
Kedzierski, Lukasz ; Escalante, Ananias A. ; Isea, Raul ; Black, Casilda G. ; Barnwell, John W. ; Coppel, Ross L. / Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase. In: Infection, Genetics and Evolution. 2002 ; Vol. 1, No. 4. pp. 297-301.
@article{4fb1e5e5a40f4442bbbea948fd09456f,
title = "Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase",
abstract = "Phylogenetic studies of the genus Plasmodium have been performed using sequences of the nuclear, mitochondrial and plastid genes. Here we have analyzed the adenylosuccinate lyase (ASL) gene, which encodes an enzyme involved in the salvage of host purines needed by malaria parasites for DNA synthesis. The ASL gene is present in several eukaryotic as well as prokaryotic organisms and does not have repeat regions, which facilitates the accuracy of the alignment. Furthermore, it has been shown that ASL is not subject to positive natural selection. We have sequenced the ASL gene of several different Plasmodium species infecting humans, rodents, monkeys and birds and used the obtained sequences along with the previously known P. falciparum ASL sequence, for structural and phylogenetic analysis of the genus Plasmodium. The genetic divergence of ASL is comparable with that observed in other nuclear genes such as cysteine proteinase, although ASL cannot be considered conserved when compared to aldolase or superoxide dismutase, which exhibit a slower rate of evolution. Nevertheless, a protein like ASL has a rate of evolution that provides enough information for elucidating evolutionary relationships. We modeled 3D structures of the ASL protein based on sequences used in the phylogenetic analysis and obtained a consistent structure for four different species despite the divergence observed. Such models would facilitate alignment in further studies with a greater number of plasmodial species or other Apicomplexa.",
keywords = "Adenylosuccinate lyase, Malaria, Phylogenetic analysis, Plasmodium",
author = "Lukasz Kedzierski and Escalante, {Ananias A.} and Raul Isea and Black, {Casilda G.} and Barnwell, {John W.} and Coppel, {Ross L.}",
year = "2002",
doi = "10.1016/S1567-1348(02)00031-X",
language = "English (US)",
volume = "1",
pages = "297--301",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Phylogenetic analysis of the genus Plasmodium based on the gene encoding adenylosuccinate lyase

AU - Kedzierski, Lukasz

AU - Escalante, Ananias A.

AU - Isea, Raul

AU - Black, Casilda G.

AU - Barnwell, John W.

AU - Coppel, Ross L.

PY - 2002

Y1 - 2002

N2 - Phylogenetic studies of the genus Plasmodium have been performed using sequences of the nuclear, mitochondrial and plastid genes. Here we have analyzed the adenylosuccinate lyase (ASL) gene, which encodes an enzyme involved in the salvage of host purines needed by malaria parasites for DNA synthesis. The ASL gene is present in several eukaryotic as well as prokaryotic organisms and does not have repeat regions, which facilitates the accuracy of the alignment. Furthermore, it has been shown that ASL is not subject to positive natural selection. We have sequenced the ASL gene of several different Plasmodium species infecting humans, rodents, monkeys and birds and used the obtained sequences along with the previously known P. falciparum ASL sequence, for structural and phylogenetic analysis of the genus Plasmodium. The genetic divergence of ASL is comparable with that observed in other nuclear genes such as cysteine proteinase, although ASL cannot be considered conserved when compared to aldolase or superoxide dismutase, which exhibit a slower rate of evolution. Nevertheless, a protein like ASL has a rate of evolution that provides enough information for elucidating evolutionary relationships. We modeled 3D structures of the ASL protein based on sequences used in the phylogenetic analysis and obtained a consistent structure for four different species despite the divergence observed. Such models would facilitate alignment in further studies with a greater number of plasmodial species or other Apicomplexa.

AB - Phylogenetic studies of the genus Plasmodium have been performed using sequences of the nuclear, mitochondrial and plastid genes. Here we have analyzed the adenylosuccinate lyase (ASL) gene, which encodes an enzyme involved in the salvage of host purines needed by malaria parasites for DNA synthesis. The ASL gene is present in several eukaryotic as well as prokaryotic organisms and does not have repeat regions, which facilitates the accuracy of the alignment. Furthermore, it has been shown that ASL is not subject to positive natural selection. We have sequenced the ASL gene of several different Plasmodium species infecting humans, rodents, monkeys and birds and used the obtained sequences along with the previously known P. falciparum ASL sequence, for structural and phylogenetic analysis of the genus Plasmodium. The genetic divergence of ASL is comparable with that observed in other nuclear genes such as cysteine proteinase, although ASL cannot be considered conserved when compared to aldolase or superoxide dismutase, which exhibit a slower rate of evolution. Nevertheless, a protein like ASL has a rate of evolution that provides enough information for elucidating evolutionary relationships. We modeled 3D structures of the ASL protein based on sequences used in the phylogenetic analysis and obtained a consistent structure for four different species despite the divergence observed. Such models would facilitate alignment in further studies with a greater number of plasmodial species or other Apicomplexa.

KW - Adenylosuccinate lyase

KW - Malaria

KW - Phylogenetic analysis

KW - Plasmodium

UR - http://www.scopus.com/inward/record.url?scp=0036301323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036301323&partnerID=8YFLogxK

U2 - 10.1016/S1567-1348(02)00031-X

DO - 10.1016/S1567-1348(02)00031-X

M3 - Article

C2 - 12798008

AN - SCOPUS:0036301323

VL - 1

SP - 297

EP - 301

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

IS - 4

ER -