Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus

Lihua Xiao, Lillian Escalante, Chunfu Yang, Irshad Sulaiman, Anannias A. Escalante, Richard J. Montali, Ronald Fayer, Altaf A. Lal

Research output: Contribution to journalArticle

507 Citations (Scopus)

Abstract

Biological data support the hypothesis that there are multiple species in the genus Cryptosporidium, but a recent analysis of the available genetic data suggested that there is insufficient evidence for species differentiation. In order to resolve the controversy in the taxonomy of this parasite genus, we characterized the small-subunit rRNA genes of Cryptosporidium parvum, Cryptosporidium baileyi, Cryptosporidium muris, and Cryptosporidium serpentis and performed a phylogenetic analysis of the genus Cryptosporidium. Our study revealed that the genus Cryptosporidium contains the phylogenetically distinct species C. parvum, C. muris, C. baileyi, and C. serpentis, which is consistent with the biological characteristics and host specificity data. The Cryptosporidium species formed two clades, with C. parvum and C. baileyi belonging to one clade and C. muris and C. serpentis belonging to the other clade. Within C. parvum, human genotype isolates and guinea pig isolates (known as Cryptosporidium wrairi) each differed from bovine genotype isolates by the nucleotide sequence in four regions. A C. muris isolate from cattle was also different from parasites isolated from a rock hyrax and a Bactrian camel. Minor differences were also detected between C. serpentis isolates from snakes and lizards. Based on the genetic information, a species- and strain-specific PCR-restriction fragment length polymorphism diagnostic tool was developed.

Original languageEnglish (US)
Pages (from-to)1578-1583
Number of pages6
JournalApplied and Environmental Microbiology
Volume65
Issue number4
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Cryptosporidium
Cryptosporidium muris
rRNA Genes
parasite
Parasites
Cryptosporidium baileyi
Cryptosporidium parvum
ribosomal RNA
phylogenetics
parasites
loci
gene
phylogeny
genes
genotype
Procaviidae
Camelus bactrianus
host specificity
biological characteristics
Hyraxes

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Xiao, L., Escalante, L., Yang, C., Sulaiman, I., Escalante, A. A., Montali, R. J., ... Lal, A. A. (1999). Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus. Applied and Environmental Microbiology, 65(4), 1578-1583.

Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus. / Xiao, Lihua; Escalante, Lillian; Yang, Chunfu; Sulaiman, Irshad; Escalante, Anannias A.; Montali, Richard J.; Fayer, Ronald; Lal, Altaf A.

In: Applied and Environmental Microbiology, Vol. 65, No. 4, 04.1999, p. 1578-1583.

Research output: Contribution to journalArticle

Xiao, L, Escalante, L, Yang, C, Sulaiman, I, Escalante, AA, Montali, RJ, Fayer, R & Lal, AA 1999, 'Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus', Applied and Environmental Microbiology, vol. 65, no. 4, pp. 1578-1583.
Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ et al. Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus. Applied and Environmental Microbiology. 1999 Apr;65(4):1578-1583.
Xiao, Lihua ; Escalante, Lillian ; Yang, Chunfu ; Sulaiman, Irshad ; Escalante, Anannias A. ; Montali, Richard J. ; Fayer, Ronald ; Lal, Altaf A. / Phylogenetic analysis of Cryptosporidium parasites based on the small- subunit rRNA gene locus. In: Applied and Environmental Microbiology. 1999 ; Vol. 65, No. 4. pp. 1578-1583.
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