TY - JOUR
T1 - Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina
AU - Du, Jianhai
AU - Rountree, Austin
AU - Cleghorn, Whitney M.
AU - Contreras, Laura
AU - Lindsay, Ken J.
AU - Sadilek, Martin
AU - Gu, Haiwei
AU - Djukovic, Danijel
AU - Raftery, Dan
AU - Satrústegui, Jorgina
AU - Kanow, Mark
AU - Chan, Lawrence
AU - Tsang, Stephen H.
AU - Sweet, Ian R.
AU - Hurley, James B.
N1 - Funding Information:
This work was supported, in whole or in part, by National Institutes of Health Grants EY06641 and EY017863 (to J. B. H.) and EY001730 (NEI Center Core). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors declare that they have no conflicts of interest with the contents of this article.
Publisher Copyright:
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2016/2/26
Y1 - 2016/2/26
N2 - Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.
AB - Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.
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U2 - 10.1074/jbc.M115.698985
DO - 10.1074/jbc.M115.698985
M3 - Article
C2 - 26677218
AN - SCOPUS:85013812932
SN - 0021-9258
VL - 291
SP - 4698
EP - 4710
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 9
ER -