Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina

Jianhai Du, Austin Rountree, Whitney M. Cleghorn, Laura Contreras, Ken J. Lindsay, Martin Sadilek, Haiwei Gu, Danijel Djukovic, Dan Raftery, Jorgina Satrústegui, Mark Kanow, Lawrence Chan, Stephen H. Tsang, Ian R. Sweet, James B. Hurley

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.

Original languageEnglish (US)
Pages (from-to)4698-4710
Number of pages13
JournalJournal of Biological Chemistry
Volume291
Issue number9
DOIs
StatePublished - Feb 26 2016
Externally publishedYes

Fingerprint

Light Signal Transduction
Metabolism
Retina
Nucleotides
Fluxes
Adenosine Triphosphate
Guanosine Monophosphate
Ketone Bodies
Purines
Production control
Citric Acid Cycle
Metabolome
Darkness
Glycolysis
Oxidoreductases
Respiration
Ions
Light

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Du, J., Rountree, A., Cleghorn, W. M., Contreras, L., Lindsay, K. J., Sadilek, M., ... Hurley, J. B. (2016). Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. Journal of Biological Chemistry, 291(9), 4698-4710. https://doi.org/10.1074/jbc.M115.698985

Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. / Du, Jianhai; Rountree, Austin; Cleghorn, Whitney M.; Contreras, Laura; Lindsay, Ken J.; Sadilek, Martin; Gu, Haiwei; Djukovic, Danijel; Raftery, Dan; Satrústegui, Jorgina; Kanow, Mark; Chan, Lawrence; Tsang, Stephen H.; Sweet, Ian R.; Hurley, James B.

In: Journal of Biological Chemistry, Vol. 291, No. 9, 26.02.2016, p. 4698-4710.

Research output: Contribution to journalArticle

Du, J, Rountree, A, Cleghorn, WM, Contreras, L, Lindsay, KJ, Sadilek, M, Gu, H, Djukovic, D, Raftery, D, Satrústegui, J, Kanow, M, Chan, L, Tsang, SH, Sweet, IR & Hurley, JB 2016, 'Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina', Journal of Biological Chemistry, vol. 291, no. 9, pp. 4698-4710. https://doi.org/10.1074/jbc.M115.698985
Du J, Rountree A, Cleghorn WM, Contreras L, Lindsay KJ, Sadilek M et al. Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. Journal of Biological Chemistry. 2016 Feb 26;291(9):4698-4710. https://doi.org/10.1074/jbc.M115.698985
Du, Jianhai ; Rountree, Austin ; Cleghorn, Whitney M. ; Contreras, Laura ; Lindsay, Ken J. ; Sadilek, Martin ; Gu, Haiwei ; Djukovic, Danijel ; Raftery, Dan ; Satrústegui, Jorgina ; Kanow, Mark ; Chan, Lawrence ; Tsang, Stephen H. ; Sweet, Ian R. ; Hurley, James B. / Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 9. pp. 4698-4710.
@article{c4398024c49c4af1a58237204e0e962d,
title = "Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina",
abstract = "Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.",
author = "Jianhai Du and Austin Rountree and Cleghorn, {Whitney M.} and Laura Contreras and Lindsay, {Ken J.} and Martin Sadilek and Haiwei Gu and Danijel Djukovic and Dan Raftery and Jorgina Satr{\'u}stegui and Mark Kanow and Lawrence Chan and Tsang, {Stephen H.} and Sweet, {Ian R.} and Hurley, {James B.}",
year = "2016",
month = "2",
day = "26",
doi = "10.1074/jbc.M115.698985",
language = "English (US)",
volume = "291",
pages = "4698--4710",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina

AU - Du, Jianhai

AU - Rountree, Austin

AU - Cleghorn, Whitney M.

AU - Contreras, Laura

AU - Lindsay, Ken J.

AU - Sadilek, Martin

AU - Gu, Haiwei

AU - Djukovic, Danijel

AU - Raftery, Dan

AU - Satrústegui, Jorgina

AU - Kanow, Mark

AU - Chan, Lawrence

AU - Tsang, Stephen H.

AU - Sweet, Ian R.

AU - Hurley, James B.

PY - 2016/2/26

Y1 - 2016/2/26

N2 - Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.

AB - Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.

UR - http://www.scopus.com/inward/record.url?scp=85013812932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013812932&partnerID=8YFLogxK

U2 - 10.1074/jbc.M115.698985

DO - 10.1074/jbc.M115.698985

M3 - Article

VL - 291

SP - 4698

EP - 4710

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 9

ER -