Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina

Jianhai Du, Austin Rountree, Whitney M. Cleghorn, Laura Contreras, Ken J. Lindsay, Martin Sadilek, Haiwei Gu, Danijel Djukovic, Dan Raftery, Jorgina Satrústegui, Mark Kanow, Lawrence Chan, Stephen H. Tsang, Ian R. Sweet, James B. Hurley

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Production of energy in a cell must keep pace with demand. Photoreceptors use ATP to maintain ion gradients in darkness, whereas in light they use it to support phototransduction. Matching production with consumption can be accomplished by coupling production directly to consumption. Alternatively, production can be set by a signal that anticipates demand. In this report we investigate the hypothesis that signaling through phototransduction controls production of energy in mouse retinas. We found that respiration in mouse retinas is not coupled tightly to ATP consumption. By analyzing metabolic flux in mouse retinas, we also found that phototransduction slows metabolic flux through glycolysis and through intermediates of the citric acid cycle. We also evaluated the relative contributions of regulation of the activities of α-ketoglutarate dehydrogenase and the aspartate-glutamate carrier 1. In addition, a comprehensive analysis of the retinal metabolome showed that phototransduction also influences steady-state concentrations of 5′-GMP, ribose-5-phosphate, ketone bodies, and purines.

Original languageEnglish (US)
Pages (from-to)4698-4710
Number of pages13
JournalJournal of Biological Chemistry
Volume291
Issue number9
DOIs
StatePublished - Feb 26 2016
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Du, J., Rountree, A., Cleghorn, W. M., Contreras, L., Lindsay, K. J., Sadilek, M., Gu, H., Djukovic, D., Raftery, D., Satrústegui, J., Kanow, M., Chan, L., Tsang, S. H., Sweet, I. R., & Hurley, J. B. (2016). Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. Journal of Biological Chemistry, 291(9), 4698-4710. https://doi.org/10.1074/jbc.M115.698985