Abstract
Monomers of Photosystem I (PS I) from the thermophilic cyanobacterium Synechococcus elongatus were prepared and characterized. Using both the zwitterionic sulfobetain 12 and the nonionic β-dodecyl maltoside as detergents, PS I was extracted in trimeric form from the thylakoid membrane. The trimers dissociate into monomers during gel-filtration, apparently due to a change in the osmotic pressure. The PS I monomer prepared in β-dodecyl maltoside exhibits the same subunit composition as the trimeric PS I. The preparation in sulfobetain 12, however, leads to the removal of the two hydrophobic subunits K and L from the monomer. Further treatment of this sample with sulfobetain 12 detaches the subunits F, J and M, associated with the disappearance of a 706 nun band in the absorption spectrum. Both the PS I monomer with complete subunit composition and the monomer lacking subunits K and L were crystallized using batch and vapor diffusion techniques with poly(ethylene glycol) as the precipitating agent.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-120 |
| Number of pages | 6 |
| Journal | BBA - Bioenergetics |
| Volume | 1229 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 4 1995 |
| Externally published | Yes |
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Keywords
- Crystallization
- Monomer preparation
- Photosystem I
- Subunit composition
- Thermophilic cyanobacterium
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
Cite this
Photosystem I from Synechococcus elongatus : preparation and crystallization of monomers with varying subunit compositions. / Jekow, Petra; Fromme, Petra; Witt, Horst T.; Saenger, Wolfram.
In: BBA - Bioenergetics, Vol. 1229, No. 1, 04.04.1995, p. 115-120.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photosystem I from Synechococcus elongatus
T2 - preparation and crystallization of monomers with varying subunit compositions
AU - Jekow, Petra
AU - Fromme, Petra
AU - Witt, Horst T.
AU - Saenger, Wolfram
PY - 1995/4/4
Y1 - 1995/4/4
N2 - Monomers of Photosystem I (PS I) from the thermophilic cyanobacterium Synechococcus elongatus were prepared and characterized. Using both the zwitterionic sulfobetain 12 and the nonionic β-dodecyl maltoside as detergents, PS I was extracted in trimeric form from the thylakoid membrane. The trimers dissociate into monomers during gel-filtration, apparently due to a change in the osmotic pressure. The PS I monomer prepared in β-dodecyl maltoside exhibits the same subunit composition as the trimeric PS I. The preparation in sulfobetain 12, however, leads to the removal of the two hydrophobic subunits K and L from the monomer. Further treatment of this sample with sulfobetain 12 detaches the subunits F, J and M, associated with the disappearance of a 706 nun band in the absorption spectrum. Both the PS I monomer with complete subunit composition and the monomer lacking subunits K and L were crystallized using batch and vapor diffusion techniques with poly(ethylene glycol) as the precipitating agent.
AB - Monomers of Photosystem I (PS I) from the thermophilic cyanobacterium Synechococcus elongatus were prepared and characterized. Using both the zwitterionic sulfobetain 12 and the nonionic β-dodecyl maltoside as detergents, PS I was extracted in trimeric form from the thylakoid membrane. The trimers dissociate into monomers during gel-filtration, apparently due to a change in the osmotic pressure. The PS I monomer prepared in β-dodecyl maltoside exhibits the same subunit composition as the trimeric PS I. The preparation in sulfobetain 12, however, leads to the removal of the two hydrophobic subunits K and L from the monomer. Further treatment of this sample with sulfobetain 12 detaches the subunits F, J and M, associated with the disappearance of a 706 nun band in the absorption spectrum. Both the PS I monomer with complete subunit composition and the monomer lacking subunits K and L were crystallized using batch and vapor diffusion techniques with poly(ethylene glycol) as the precipitating agent.
KW - Crystallization
KW - Monomer preparation
KW - Photosystem I
KW - Subunit composition
KW - Thermophilic cyanobacterium
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UR - http://www.scopus.com/inward/citedby.url?scp=0028956304&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(94)00201-F
DO - 10.1016/0005-2728(94)00201-F
M3 - Article
VL - 1229
SP - 115
EP - 120
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
ER -