TY - JOUR
T1 - Photosystem I from Synechococcus elongatus
T2 - preparation and crystallization of monomers with varying subunit compositions
AU - Jekow, Petra
AU - Fromme, Petra
AU - Witt, Horst T.
AU - Saenger, Wolfram
N1 - Funding Information:
We thank D. di Fiore, C. Otto and A. F~Srstefro r technicaslu pporat ndW.-D. Schuberfto r carefullyre ading the manuscripTt.h is work was supportebdy the Deutsche Forschungsgemeinscthharfot ughS onderforschungsbereich 312 (H.T.W. and W.S.) and by Fonds der Chemischen Industrie(H .T.W. and W.S.)
PY - 1995/4/4
Y1 - 1995/4/4
N2 - Monomers of Photosystem I (PS I) from the thermophilic cyanobacterium Synechococcus elongatus were prepared and characterized. Using both the zwitterionic sulfobetain 12 and the nonionic β-dodecyl maltoside as detergents, PS I was extracted in trimeric form from the thylakoid membrane. The trimers dissociate into monomers during gel-filtration, apparently due to a change in the osmotic pressure. The PS I monomer prepared in β-dodecyl maltoside exhibits the same subunit composition as the trimeric PS I. The preparation in sulfobetain 12, however, leads to the removal of the two hydrophobic subunits K and L from the monomer. Further treatment of this sample with sulfobetain 12 detaches the subunits F, J and M, associated with the disappearance of a 706 nun band in the absorption spectrum. Both the PS I monomer with complete subunit composition and the monomer lacking subunits K and L were crystallized using batch and vapor diffusion techniques with poly(ethylene glycol) as the precipitating agent.
AB - Monomers of Photosystem I (PS I) from the thermophilic cyanobacterium Synechococcus elongatus were prepared and characterized. Using both the zwitterionic sulfobetain 12 and the nonionic β-dodecyl maltoside as detergents, PS I was extracted in trimeric form from the thylakoid membrane. The trimers dissociate into monomers during gel-filtration, apparently due to a change in the osmotic pressure. The PS I monomer prepared in β-dodecyl maltoside exhibits the same subunit composition as the trimeric PS I. The preparation in sulfobetain 12, however, leads to the removal of the two hydrophobic subunits K and L from the monomer. Further treatment of this sample with sulfobetain 12 detaches the subunits F, J and M, associated with the disappearance of a 706 nun band in the absorption spectrum. Both the PS I monomer with complete subunit composition and the monomer lacking subunits K and L were crystallized using batch and vapor diffusion techniques with poly(ethylene glycol) as the precipitating agent.
KW - Crystallization
KW - Monomer preparation
KW - Photosystem I
KW - Subunit composition
KW - Thermophilic cyanobacterium
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U2 - 10.1016/0005-2728(94)00201-F
DO - 10.1016/0005-2728(94)00201-F
M3 - Article
AN - SCOPUS:0028956304
VL - 1229
SP - 115
EP - 120
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
ER -