Photosynthetic electron transport involved in PxcA-dependent proton extrusion in Synechocystis sp. strain PCC6803

Effect of pxcA inactivation on CO2, HCO3 -, and NO3 - uptake

Masatoshi Sonoda, Hirokazu Katoh, Willem Vermaas, George Schmetterer, Teruo Ogawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The product of pxcA (formerly known as cotA) is involved in light- induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p- benzoquinone, net proton extrusion by Synechocystis sp. strain PCC6803 ceased after 1 min of illumination and a postillumination influx of protons was observed, suggesting that the PxcA-dependent, light-dependent proton extrusion equilibrates with a light-independent influx of protons. A photosystem I (PS I) deletion mutant extruded a large number of protons in the light. Thus, PS II-dependent electron transfer and proton translocation are major factors in light-driven proton extrusion, presumably mediated by ATP synthesis. Inhibition of CO2 fixation by glyceraldehyde in a cytochrome c oxidase (COX) deletion mutant strongly inhibited the proton extrusion. Leakage of PS II-generated electrons to oxygen via COX appears to be required for proton extrusion when CO2 fixation is inhibited. At pH 8.0, NO3 - uptake activity was very low in the pxcA mutant at low [Na+] (~100 μM). At pH 6.5, the pxcA strain did not take up CO2 or NO3 - at low [Na+] and showed very low CO2 uptake activity even at 15 mM Na+. A possible role of PxcA-dependent proton exchange in charge and pH homeostasis during uptake of CO2, HCO3 -, and NO3 - is discussed.

Original languageEnglish (US)
Pages (from-to)3799-3803
Number of pages5
JournalJournal of Bacteriology
Volume180
Issue number15
StatePublished - Aug 1998

Fingerprint

Synechocystis
Electron Transport
Protons
Light
Electrons
Glyceraldehyde
Photosystem I Protein Complex
Electron Transport Complex IV
Lighting
Oxidoreductases
Homeostasis
Adenosine Triphosphate

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Photosynthetic electron transport involved in PxcA-dependent proton extrusion in Synechocystis sp. strain PCC6803 : Effect of pxcA inactivation on CO2, HCO3 -, and NO3 - uptake. / Sonoda, Masatoshi; Katoh, Hirokazu; Vermaas, Willem; Schmetterer, George; Ogawa, Teruo.

In: Journal of Bacteriology, Vol. 180, No. 15, 08.1998, p. 3799-3803.

Research output: Contribution to journalArticle

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abstract = "The product of pxcA (formerly known as cotA) is involved in light- induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p- benzoquinone, net proton extrusion by Synechocystis sp. strain PCC6803 ceased after 1 min of illumination and a postillumination influx of protons was observed, suggesting that the PxcA-dependent, light-dependent proton extrusion equilibrates with a light-independent influx of protons. A photosystem I (PS I) deletion mutant extruded a large number of protons in the light. Thus, PS II-dependent electron transfer and proton translocation are major factors in light-driven proton extrusion, presumably mediated by ATP synthesis. Inhibition of CO2 fixation by glyceraldehyde in a cytochrome c oxidase (COX) deletion mutant strongly inhibited the proton extrusion. Leakage of PS II-generated electrons to oxygen via COX appears to be required for proton extrusion when CO2 fixation is inhibited. At pH 8.0, NO3 - uptake activity was very low in the pxcA mutant at low [Na+] (~100 μM). At pH 6.5, the pxcA strain did not take up CO2 or NO3 - at low [Na+] and showed very low CO2 uptake activity even at 15 mM Na+. A possible role of PxcA-dependent proton exchange in charge and pH homeostasis during uptake of CO2, HCO3 -, and NO3 - is discussed.",
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T2 - Effect of pxcA inactivation on CO2, HCO3 -, and NO3 - uptake

AU - Sonoda, Masatoshi

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N2 - The product of pxcA (formerly known as cotA) is involved in light- induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p- benzoquinone, net proton extrusion by Synechocystis sp. strain PCC6803 ceased after 1 min of illumination and a postillumination influx of protons was observed, suggesting that the PxcA-dependent, light-dependent proton extrusion equilibrates with a light-independent influx of protons. A photosystem I (PS I) deletion mutant extruded a large number of protons in the light. Thus, PS II-dependent electron transfer and proton translocation are major factors in light-driven proton extrusion, presumably mediated by ATP synthesis. Inhibition of CO2 fixation by glyceraldehyde in a cytochrome c oxidase (COX) deletion mutant strongly inhibited the proton extrusion. Leakage of PS II-generated electrons to oxygen via COX appears to be required for proton extrusion when CO2 fixation is inhibited. At pH 8.0, NO3 - uptake activity was very low in the pxcA mutant at low [Na+] (~100 μM). At pH 6.5, the pxcA strain did not take up CO2 or NO3 - at low [Na+] and showed very low CO2 uptake activity even at 15 mM Na+. A possible role of PxcA-dependent proton exchange in charge and pH homeostasis during uptake of CO2, HCO3 -, and NO3 - is discussed.

AB - The product of pxcA (formerly known as cotA) is involved in light- induced Na+-dependent proton extrusion. In the presence of 2,5-dimethyl-p- benzoquinone, net proton extrusion by Synechocystis sp. strain PCC6803 ceased after 1 min of illumination and a postillumination influx of protons was observed, suggesting that the PxcA-dependent, light-dependent proton extrusion equilibrates with a light-independent influx of protons. A photosystem I (PS I) deletion mutant extruded a large number of protons in the light. Thus, PS II-dependent electron transfer and proton translocation are major factors in light-driven proton extrusion, presumably mediated by ATP synthesis. Inhibition of CO2 fixation by glyceraldehyde in a cytochrome c oxidase (COX) deletion mutant strongly inhibited the proton extrusion. Leakage of PS II-generated electrons to oxygen via COX appears to be required for proton extrusion when CO2 fixation is inhibited. At pH 8.0, NO3 - uptake activity was very low in the pxcA mutant at low [Na+] (~100 μM). At pH 6.5, the pxcA strain did not take up CO2 or NO3 - at low [Na+] and showed very low CO2 uptake activity even at 15 mM Na+. A possible role of PxcA-dependent proton exchange in charge and pH homeostasis during uptake of CO2, HCO3 -, and NO3 - is discussed.

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