Photolysis and Recombination of Adenosylcobalamin Bound to Glutamate Mutase

Roseanne J. Sension, Allwyn G. Cole, Ahmasi D. Harris, Christel C. Fox, Neal Woodbury, Su Lin, E. Neil G Marsh

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Ultrafast spectroscopic measurements are used to determine the kinetics of homolysis and recombination for adenosylcobalamin bound in the active site of glutamate mutase. These are the first such measurements on an adenosylcobalamin-dependent enzyme. A short-lived intermediate is formed prior to formation of the cob(II)alamin radical. This intermediate was not observed upon photolysis of adenosylcobalamin in free solution. The intrinsic rate constant for geminate recombination for adenosylcobalamin bound to glutamate mutase is 1.08 ± 0.10 ns-1, only 16% smaller than the rate constant measured in free solution, 1.39 ± 0.06 ns-1, suggesting the protein does not greatly perturb the stability of the cobalt-carbon bond upon binding the coenzyme.

Original languageEnglish (US)
Pages (from-to)1598-1599
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number6
StatePublished - Feb 18 2004

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methylaspartate mutase
Photolysis
Genetic Recombination
Rate constants
Coenzymes
Cobalt
Enzymes
Proteins
Kinetics
Carbon
Catalytic Domain
cobamamide

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Sension, R. J., Cole, A. G., Harris, A. D., Fox, C. C., Woodbury, N., Lin, S., & Marsh, E. N. G. (2004). Photolysis and Recombination of Adenosylcobalamin Bound to Glutamate Mutase. Journal of the American Chemical Society, 126(6), 1598-1599.

Photolysis and Recombination of Adenosylcobalamin Bound to Glutamate Mutase. / Sension, Roseanne J.; Cole, Allwyn G.; Harris, Ahmasi D.; Fox, Christel C.; Woodbury, Neal; Lin, Su; Marsh, E. Neil G.

In: Journal of the American Chemical Society, Vol. 126, No. 6, 18.02.2004, p. 1598-1599.

Research output: Contribution to journalArticle

Sension, RJ, Cole, AG, Harris, AD, Fox, CC, Woodbury, N, Lin, S & Marsh, ENG 2004, 'Photolysis and Recombination of Adenosylcobalamin Bound to Glutamate Mutase', Journal of the American Chemical Society, vol. 126, no. 6, pp. 1598-1599.
Sension, Roseanne J. ; Cole, Allwyn G. ; Harris, Ahmasi D. ; Fox, Christel C. ; Woodbury, Neal ; Lin, Su ; Marsh, E. Neil G. / Photolysis and Recombination of Adenosylcobalamin Bound to Glutamate Mutase. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 6. pp. 1598-1599.
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