Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells

Amy R. Donaldson, Constantin Edi Tanase, Dennis Awuah, Pranav Vasanthi Bathrinarayanan, Laurence Hall, Mehdi Nikkhah, Ali Khademhosseini, Felicity Rose, Cameron Alexander, Amir M. Ghaemmaghami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned depending on the specific application. There is a growing appreciation for the interaction between biomaterials and cells of the immune system with the increasing usage of biomaterials for in vivo applications. Here, we addressed the current lack of information regarding the immune-modulatory properties of photocrosslinked GelMA. We investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform. Using lipopolysaccharide to stimulate a pro-inflammatory immune response, we found tumor necrosis factor-α (TNF-α) expression was suppressed in GelMA culture conditions. Our findings have important implications on the future use of GelMA, and potentially similar hydrogels, and highlight the significance of investigating the potential immune-modulatory properties of biomaterials.

Original languageEnglish (US)
Article number116
JournalFrontiers in Bioengineering and Biotechnology
Volume6
Issue numberSEP
DOIs
StatePublished - Sep 19 2018

Fingerprint

Hydrogels
Gelatin
Biomaterials
Biocompatible Materials
Cytokines
Tissue engineering
Tissue Engineering
Immune system
Drug delivery
Cell culture
Hydrogel
Physical properties
Lipopolysaccharides
Immune System
Cell Culture Techniques
Tumor Necrosis Factor-alpha
human TNF protein
Pharmaceutical Preparations

Keywords

  • Biomaterials
  • Gelatin methacryloyl
  • Immunology
  • Tissue engineering
  • TNF-α

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Cite this

Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. / Donaldson, Amy R.; Tanase, Constantin Edi; Awuah, Dennis; Bathrinarayanan, Pranav Vasanthi; Hall, Laurence; Nikkhah, Mehdi; Khademhosseini, Ali; Rose, Felicity; Alexander, Cameron; Ghaemmaghami, Amir M.

In: Frontiers in Bioengineering and Biotechnology, Vol. 6, No. SEP, 116, 19.09.2018.

Research output: Contribution to journalArticle

Donaldson, AR, Tanase, CE, Awuah, D, Bathrinarayanan, PV, Hall, L, Nikkhah, M, Khademhosseini, A, Rose, F, Alexander, C & Ghaemmaghami, AM 2018, 'Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells', Frontiers in Bioengineering and Biotechnology, vol. 6, no. SEP, 116. https://doi.org/10.3389/fbioe.2018.00116
Donaldson, Amy R. ; Tanase, Constantin Edi ; Awuah, Dennis ; Bathrinarayanan, Pranav Vasanthi ; Hall, Laurence ; Nikkhah, Mehdi ; Khademhosseini, Ali ; Rose, Felicity ; Alexander, Cameron ; Ghaemmaghami, Amir M. / Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. In: Frontiers in Bioengineering and Biotechnology. 2018 ; Vol. 6, No. SEP.
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