Photoacoustic Flow System for the Detection of Ovarian Circulating Tumor Cells Utilizing Copper Sulfide Nanoparticles

Joel F. Lusk, Christopher Miranda, Madeleine Howell, Matthew Chrest, Jarrett Eshima, Barbara Smith

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The development of cell-specific photoacoustic (PA) contrast agents within systems of fluidic flow provides opportunities for the accurate detection of early stage cancer metastasis. Despite the promise of exogenous contrast agents for use in clinical settings, applications are currently limited by both material biocompatibility and target specificity. In this study, folic acid functionalized copper sulfide nanoparticles (FA-CuS NPs) are synthesized to enable ovarian-cancer-specific binding and PA detection in a custom flow system. Folate receptors, known to be overexpressed on the surface of ovarian cancer cells, have remained an ideal candidate for specific targeting through functionalization on nanoparticles and other contrast agents. In combination with copper sulfide nanoparticles' strong absorbance in the near-infrared (NIR), these FA-CuS NPs are an ideal contrast agent capable of being detected by photoacoustic flow cytometry. For the first time, this study shows a potential PA contrast agent to accurately identify ovarian circulating tumor cells in flow.

Original languageEnglish (US)
Pages (from-to)1553-1560
Number of pages8
JournalACS Biomaterials Science and Engineering
Volume5
Issue number3
DOIs
StatePublished - Mar 11 2019

Fingerprint

Photoacoustic effect
Sulfides
Contrast Media
Tumors
Copper
Cells
Nanoparticles
Folic Acid
Time and motion study
Acids
Flow cytometry
Fluidics
Biocompatibility
Infrared radiation

Keywords

  • circulating tumor cells
  • copper sulfide nanoparticles
  • ovarian cancer
  • photoacoustic contrast agents
  • photoacoustic flow cytometry

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Photoacoustic Flow System for the Detection of Ovarian Circulating Tumor Cells Utilizing Copper Sulfide Nanoparticles. / Lusk, Joel F.; Miranda, Christopher; Howell, Madeleine; Chrest, Matthew; Eshima, Jarrett; Smith, Barbara.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 3, 11.03.2019, p. 1553-1560.

Research output: Contribution to journalArticle

Lusk, Joel F. ; Miranda, Christopher ; Howell, Madeleine ; Chrest, Matthew ; Eshima, Jarrett ; Smith, Barbara. / Photoacoustic Flow System for the Detection of Ovarian Circulating Tumor Cells Utilizing Copper Sulfide Nanoparticles. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 3. pp. 1553-1560.
@article{cb78183f6c624bc7911929fbe457cb20,
title = "Photoacoustic Flow System for the Detection of Ovarian Circulating Tumor Cells Utilizing Copper Sulfide Nanoparticles",
abstract = "The development of cell-specific photoacoustic (PA) contrast agents within systems of fluidic flow provides opportunities for the accurate detection of early stage cancer metastasis. Despite the promise of exogenous contrast agents for use in clinical settings, applications are currently limited by both material biocompatibility and target specificity. In this study, folic acid functionalized copper sulfide nanoparticles (FA-CuS NPs) are synthesized to enable ovarian-cancer-specific binding and PA detection in a custom flow system. Folate receptors, known to be overexpressed on the surface of ovarian cancer cells, have remained an ideal candidate for specific targeting through functionalization on nanoparticles and other contrast agents. In combination with copper sulfide nanoparticles' strong absorbance in the near-infrared (NIR), these FA-CuS NPs are an ideal contrast agent capable of being detected by photoacoustic flow cytometry. For the first time, this study shows a potential PA contrast agent to accurately identify ovarian circulating tumor cells in flow.",
keywords = "circulating tumor cells, copper sulfide nanoparticles, ovarian cancer, photoacoustic contrast agents, photoacoustic flow cytometry",
author = "Lusk, {Joel F.} and Christopher Miranda and Madeleine Howell and Matthew Chrest and Jarrett Eshima and Barbara Smith",
year = "2019",
month = "3",
day = "11",
doi = "10.1021/acsbiomaterials.8b01217",
language = "English (US)",
volume = "5",
pages = "1553--1560",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Photoacoustic Flow System for the Detection of Ovarian Circulating Tumor Cells Utilizing Copper Sulfide Nanoparticles

AU - Lusk, Joel F.

AU - Miranda, Christopher

AU - Howell, Madeleine

AU - Chrest, Matthew

AU - Eshima, Jarrett

AU - Smith, Barbara

PY - 2019/3/11

Y1 - 2019/3/11

N2 - The development of cell-specific photoacoustic (PA) contrast agents within systems of fluidic flow provides opportunities for the accurate detection of early stage cancer metastasis. Despite the promise of exogenous contrast agents for use in clinical settings, applications are currently limited by both material biocompatibility and target specificity. In this study, folic acid functionalized copper sulfide nanoparticles (FA-CuS NPs) are synthesized to enable ovarian-cancer-specific binding and PA detection in a custom flow system. Folate receptors, known to be overexpressed on the surface of ovarian cancer cells, have remained an ideal candidate for specific targeting through functionalization on nanoparticles and other contrast agents. In combination with copper sulfide nanoparticles' strong absorbance in the near-infrared (NIR), these FA-CuS NPs are an ideal contrast agent capable of being detected by photoacoustic flow cytometry. For the first time, this study shows a potential PA contrast agent to accurately identify ovarian circulating tumor cells in flow.

AB - The development of cell-specific photoacoustic (PA) contrast agents within systems of fluidic flow provides opportunities for the accurate detection of early stage cancer metastasis. Despite the promise of exogenous contrast agents for use in clinical settings, applications are currently limited by both material biocompatibility and target specificity. In this study, folic acid functionalized copper sulfide nanoparticles (FA-CuS NPs) are synthesized to enable ovarian-cancer-specific binding and PA detection in a custom flow system. Folate receptors, known to be overexpressed on the surface of ovarian cancer cells, have remained an ideal candidate for specific targeting through functionalization on nanoparticles and other contrast agents. In combination with copper sulfide nanoparticles' strong absorbance in the near-infrared (NIR), these FA-CuS NPs are an ideal contrast agent capable of being detected by photoacoustic flow cytometry. For the first time, this study shows a potential PA contrast agent to accurately identify ovarian circulating tumor cells in flow.

KW - circulating tumor cells

KW - copper sulfide nanoparticles

KW - ovarian cancer

KW - photoacoustic contrast agents

KW - photoacoustic flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=85062788577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062788577&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.8b01217

DO - 10.1021/acsbiomaterials.8b01217

M3 - Article

AN - SCOPUS:85062788577

VL - 5

SP - 1553

EP - 1560

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 3

ER -