Abstract
α-Actinin is a cell-adhesion and cytoskeletal protein that bundles actin microfilaments and links these filaments directly to integrin-adhesion receptors. Phosphoinositides bind to and regulate the interaction of α-actinin with actin filaments and integrin receptors. In the present study, we demonstrate that PtdIns(3,4,5)P3 inhibits and disrupts α-actinin-bundling activity, whereas PtdIns(4,5)P2 can only inhibit activity. In addition, a protease-sensitivity assay was developed to examine the flexibility of the linker region between the actin-binding domain and the spectrin repeats of α-actinin. Both phosphoinositides influenced the extent of proteolysis and the cleavage sites. PtdIns(4,5)P2 binding decreased the proteolysis of α-actinin, suggesting a role in stabilizing the structure of the protein. In contrast, PtdIns(SAS)P3 binding enhanced α-actinin proteolysis, indicating an increase in the flexibility of the protein. Furthermore, phosphoinositide binding influenced the proteolysis of the N- and C-terminal domains of α-actinin, indicating regulation of structure within both domains. These results support the hypothesis that PtdIns(4,5)P2 and PtdIns(SAS)P3 differentially regulate α-actinin function by modulating the structure and flexibility of the protein.
Original language | English (US) |
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Pages (from-to) | 1067-1072 |
Number of pages | 6 |
Journal | Biochemical Journal |
Volume | 378 |
Issue number | 3 |
DOIs | |
State | Published - Mar 15 2004 |
Externally published | Yes |
Keywords
- Actin bundling
- Cytoskeleton
- Focal adhesion
- Phosphoinositide
- Protein flexibility
- α-actinin
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology