TY - JOUR
T1 - Phosphate depletion controls lipid content and accumulation of heterotrophic bacteria during growth of Synechocystis sp. PCC 6803
AU - Zhou, Yun
AU - Lai, Yen Jung Sean
AU - Eustance, Everett
AU - Xia, Siqing
AU - Rittmann, Bruce
N1 - Funding Information:
Funding This work was supported by LightWorks, Arizona State University, and in part by the National Science Foundation of China (51678422, 51378368), the National Key Project of Research and Development Plan of China (No. 2017YFC0403400) and Shanghai Tongji Gao Tingyao Environmental Science and Technology Development Foundation.
Funding Information:
We thank Dr. Dong Fu at the Center of Infectious Diseases and Vaccinology, Biodesign Institute at Arizona State University, for her expertise in flow cytometry for sample quantification; and Dr. Willem Vermaas and his laboratory in the School of Life Sciences at Arizona State University for providing Synechocystis sp. PCC6803.
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/6/18
Y1 - 2019/6/18
N2 - During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.
AB - During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.
KW - Batch experiment
KW - Heterotrophic bacteria
KW - Microbial community
KW - Phosphate
KW - Synechocystis sp. PCC 6803
UR - http://www.scopus.com/inward/record.url?scp=85065015660&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065015660&partnerID=8YFLogxK
U2 - 10.1007/s00253-019-09817-9
DO - 10.1007/s00253-019-09817-9
M3 - Article
C2 - 31030288
AN - SCOPUS:85065015660
SN - 0175-7598
VL - 103
SP - 5007
EP - 5014
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 12
ER -