Abstract

During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.

Original languageEnglish (US)
JournalApplied Microbiology and Biotechnology
DOIs
StatePublished - Jan 1 2019

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Synechocystis
Cyanobacteria
Biomass
Phosphates
Bacteria
Lipids
Growth
Polymers
Food

Keywords

  • Batch experiment
  • Heterotrophic bacteria
  • Microbial community
  • Phosphate
  • Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Phosphate depletion controls lipid content and accumulation of heterotrophic bacteria during growth of Synechocystis sp. PCC 6803. / Zhou, Yun; Lai, Yen Jung Sean; Eustance, Everett; Xia, Siqing; Rittmann, Bruce.

In: Applied Microbiology and Biotechnology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.",
keywords = "Batch experiment, Heterotrophic bacteria, Microbial community, Phosphate, Synechocystis sp. PCC 6803",
author = "Yun Zhou and Lai, {Yen Jung Sean} and Everett Eustance and Siqing Xia and Bruce Rittmann",
year = "2019",
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AU - Zhou, Yun

AU - Lai, Yen Jung Sean

AU - Eustance, Everett

AU - Xia, Siqing

AU - Rittmann, Bruce

PY - 2019/1/1

Y1 - 2019/1/1

N2 - During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.

AB - During the culturing of cyanobacteria, heterotrophic bacteria can compete for nutrients, compromise the quality of the harvested biomass, or cause culture crashes. We systematically investigated the effects of depleting inorganic phosphate (Pi) on the growth of the cyanobacterium Synechocystis sp. PCC 6803, its community of heterotrophic bacteria, and the biomass’s chemical composition. On the one hand, depleting Pi had minimal impact on total biomass, extracellular polymeric substances (ESP), soluble microbial products (SMP), and most types of intracellular organic polymers production. On the other hand, depleting Pi led to markedly less lipid content, less heterotrophic biomass, and a shift in the heterotrophic community from Burkholderiales to Sphingobacteriales and Saprospirales. The causes of the large impacts were that Synechocystis was much better at scavenging a very low Pi concentration and lowering the Pi available to the heterotrophs. This work lays a foundation for controlling the accumulation of heterotrophs and reducing their deleterious effects in cyanobacteria culturing.

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