Phenotypic conversion of drug-resistant bacteria to drug sensitivity

Cecilia Guerrier-Takada, Reza Salavati, Sidney Altman

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic resistance genes. The EGSs direct RNase P to cleave the mRNAs transcribed from these genes thereby converting the phenotype of drug-resistant cells to drug sensitivity. Increasing the EGS-to-target mRNA ratio by changing gene copy number or the number of EGSs complementary to different target sites enhances the efficiency of the conversion process. We demonstrate a general method for the efficient phenotypic conversion of drug-resistant bacterial cultures.

Original languageEnglish (US)
Pages (from-to)8468-8472
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number16
DOIs
StatePublished - Aug 5 1997
Externally publishedYes

Fingerprint

Bacteria
Oligoribonucleotides
Ribonuclease P
Pharmaceutical Preparations
Synthetic Genes
Messenger RNA
Gene Dosage
Microbial Drug Resistance
Genes
Plasmids
Escherichia coli
Phenotype

Keywords

  • Bacterial drug resistance
  • External guide sequences
  • RNase P

ASJC Scopus subject areas

  • General

Cite this

Phenotypic conversion of drug-resistant bacteria to drug sensitivity. / Guerrier-Takada, Cecilia; Salavati, Reza; Altman, Sidney.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 16, 05.08.1997, p. 8468-8472.

Research output: Contribution to journalArticle

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