Abstract
In a survey of the active components of crude plant extracts for their ability to cleave DNA, a crude extract prepared from Piper caninum was found to induce the relaxation of supercoiled pBR322 plasmid DNA in the presence of Cu2+. Bioassay-guided fractionation was carried out on this extract, guided by an in vitro DNA strand scission assay. Three active principles were isolated and identified as N-cis-feruloyl tyramine (1),N-trans-feruloyl tyramine (2), and 1-cinnamoylpyrrolidine (3). Compounds 1-3 represent a structurally new type of DNA strand scission agent.
Original language | English (US) |
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Pages (from-to) | 3885-3889 |
Number of pages | 5 |
Journal | Bioorganic and Medicinal Chemistry |
Volume | 12 |
Issue number | 14 |
DOIs | |
State | Published - Jul 15 2004 |
Externally published | Yes |
Keywords
- Chemotherapy
- Natural products
- Nucleic acids
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry