Phasing coherently illuminated nanocrystals bounded by partial unit cells

Richard Kirian, Richard J. Bean, Kenneth R. Beyerlein, Oleksandr M. Yefanov, Thomas A. White, Anton Barty, Henry N. Chapman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

With the use of highly coherent femtosecond X-ray pulses from a free-electron laser, it is possible to record protein nanocrystal diffraction patterns with far more information than is present in conventional crystallographic diffraction data. It has been suggested that diffraction phases may be retrieved from such data via iterative algorithms, without the use of a priori information and without restrictions on resolution. Here, we investigate the extension of this approach to nanocrystals with edge terminations that produce partial unit cells, and hence cannot be described by a common repeating unit cell. In this situation, the phase problemdescribed in previouswork must be reformulated. We demonstrate an approximate solution to this phase problem for crystals with random edge terminations.

Original languageEnglish (US)
Article number20130331
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume369
Issue number1647
DOIs
StatePublished - Jul 17 2014
Externally publishedYes

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nanocrystals
Nanoparticles
Nanocrystals
diffraction
Diffraction
Free electron lasers
Diffraction patterns
lasers
crystals
Laser pulses
X-radiation
Lasers
electrons
X-Rays
cells
Electrons
X rays
Crystals
laser
crystal

Keywords

  • Coherent diffractive imaging
  • Free-electron laser
  • Protein crystallography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Phasing coherently illuminated nanocrystals bounded by partial unit cells. / Kirian, Richard; Bean, Richard J.; Beyerlein, Kenneth R.; Yefanov, Oleksandr M.; White, Thomas A.; Barty, Anton; Chapman, Henry N.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, No. 1647, 20130331, 17.07.2014.

Research output: Contribution to journalArticle

Kirian, Richard ; Bean, Richard J. ; Beyerlein, Kenneth R. ; Yefanov, Oleksandr M. ; White, Thomas A. ; Barty, Anton ; Chapman, Henry N. / Phasing coherently illuminated nanocrystals bounded by partial unit cells. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 2014 ; Vol. 369, No. 1647.
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