Pharmacological characterization of central and peripheral type I and type II adrenal steroid receptors in the prairie vole, a glucocorticoid-resistant rodent

N. B. Hastings, Miles Orchinik, M. V. Aubourg, B. S. McEwen

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19 Citations (Scopus)

Abstract

The prairie vole (Microtus ochrogaster) has recently been shown to be glucocorticoid resistant; that is, the prairie vole adrenal axis is refractory to dexamethasone challenge, and highly elevated basal corticosterone titers occur without apparent pathophysiology. This study investigates the physiological correlates of glucocorticoid resistance in the prairie vole. We provide a detailed pharmacological characterization of intracellular type I and type II adrenal steroid receptors in peripheral tissues and the hippocampus of the prairie vole and the Sprague Dawley rat, a corticosensitive rodent. Adrenalectomy markedly reduces, but does not eliminate, circulating glucocorticoids in the prairie vole. Nonetheless, molecular, cellular, and physiological assays indicate adrenal insufficiency; salt appetite and dentate gyrus granule cell death are increased after adrenalectomy, suggesting vacancy of the high affinity type I subtype of central adrenal steroid receptor. Analysis of adrenal steroid receptor binding constants and selectivity for endogenous and synthetic steroids in the vole and rat indicated that the vole type I receptor is nearly identical to that of the rat in brain and periphery. However, voles demonstrated a 2-fold lower type I receptor binding density in colon and hippocampus compared with that in rats. The vole type II receptor bound the endogenous glucocorticoid corticosterone with an 8- to 10-fold lower affinity than the rat type II receptor and was expressed in lower densities in thymus and hippocampus. These data indicate physiological adaptations in the prairie vole adrenal axis consistent with other glucocorticoid-resistant species, such as the guinea pig and squirrel monkey.

Original languageEnglish (US)
Pages (from-to)4459-4469
Number of pages11
JournalEndocrinology
Volume140
Issue number10
StatePublished - 1999
Externally publishedYes

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Arvicolinae
Steroid Receptors
Glucocorticoids
Rodentia
Pharmacology
Hippocampus
Adrenalectomy
Corticosterone
Grassland
Physiological Adaptation
Saimiri
Adrenal Insufficiency
Dentate Gyrus
Appetite
Thymus Gland
Dexamethasone
Sprague Dawley Rats
Guinea Pigs
Colon
Cell Death

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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Pharmacological characterization of central and peripheral type I and type II adrenal steroid receptors in the prairie vole, a glucocorticoid-resistant rodent. / Hastings, N. B.; Orchinik, Miles; Aubourg, M. V.; McEwen, B. S.

In: Endocrinology, Vol. 140, No. 10, 1999, p. 4459-4469.

Research output: Contribution to journalArticle

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