Abstract

In situ bioreduction of soluble hexavalent uranium U(VI) to insoluble U(IV) (as UO2) has been proposed as a means of preventing U migration in the groundwater. This work focuses on the bioreduction of U(VI) and precipitation of U(IV). It uses anaerobic batch reactors with Desulfovibrio vulgaris, a well-known sulfate, iron, and U(VI) reducer, growing on lactate as the electron donor, in the absence of sulfate, and with a 30-mM bicarbonate buffering. In the absence of sulfate, D. vulgaris reduced >90% of the total soluble U(VI) (1 mM) to form U(IV) solids that were characterized by X-ray diffraction and confirmed to be nano-crystalline uraninite with crystallite size 2.8 ± 0.2 nm. pH values between 6 and 10 had minimal impact on bacterial growth and end-product distribution, supporting that the mono-nuclear, and poly-nuclear forms of U(VI) were equally bioavailable as electron acceptors. Electron balances support that H2 transiently accumulated, but was ultimately oxidized via U(VI) respiration. Thus, D. vulgaris utilized H2 as the electron carrier to drive respiration of U(VI). Rapid lactate utilization and biomass growth occurred only when U(VI) respiration began to draw down the sink of H2 and relieve thermodynamic inhibition of fermentation.

Original languageEnglish (US)
Pages (from-to)1465-1474
Number of pages10
JournalBiotechnology and Bioengineering
Volume115
Issue number6
DOIs
StatePublished - Jun 1 2018

Fingerprint

Desulfovibrio vulgaris
Hydrogen
Respiration
Electrons
Sulfates
Lactic Acid
Enzyme inhibition
Uranium
Groundwater
Batch reactors
Crystallite size
Bicarbonates
Growth
Thermodynamics
X-Ray Diffraction
Biomass
Fermentation
Iron
Crystalline materials
X ray diffraction

Keywords

  • bioavailable U(VI) species
  • Desulfovibrio vulgaris
  • hydrogen gas
  • U(VI) reduction
  • UO nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

pH-dependent speciation and hydrogen (H2) control U(VI) respiration by Desulfovibrio vulgaris. / Kushwaha, Shilpi; Marcus, Andrew; Rittmann, Bruce.

In: Biotechnology and Bioengineering, Vol. 115, No. 6, 01.06.2018, p. 1465-1474.

Research output: Contribution to journalArticle

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