TY - JOUR
T1 - PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues
AU - Kharaziha, Mahshid
AU - Nikkhah, Mehdi
AU - Shin, Su Ryon
AU - Annabi, Nasim
AU - Masoumi, Nafiseh
AU - Gaharwar, Akhilesh K.
AU - Camci-Unal, Gulden
AU - Khademhosseini, Ali
N1 - Funding Information:
The authors acknowledge funding from the Presidential Early Career Award for Scientists and Engineers (PECASE) , the Office of Naval Research Young National Investigator Award , the National Science Foundation CAREER Award ( DMR 0847287 ), and the National Institutes of Health ( HL092836 , AR057837 , DE021468 , DE019024 , EB012597 , HL099073 , EB008392 ) and MIT-Portugal Program (MPP-09Call-Langer-47).
PY - 2013/9
Y1 - 2013/9
N2 - A significant challenge in cardiac tissue engineering is the development of biomimetic grafts that can potentially promote myocardial repair and regeneration. A number of approaches have used engineered scaffolds to mimic the architecture of the native myocardium tissue and precisely regulate cardiac cell functions. However, previous attempts have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the myocardial extracellular matrix (ECM). In this study, we utilized an electrospinning approach to fabricate elastomeric biodegradable poly(glycerol sebacate) (PGS):gelatin nanofibrous scaffolds with a wide range of chemical composition, stiffness and anisotropy. Our findings demonstrated that through incorporation of PGS, it is possible to create nanofibrous scaffolds with well-defined anisotropy that mimic the left ventricular myocardium architecture. Furthermore, we studied attachment, proliferation, differentiation and alignment of neonatal rat cardiac fibroblast cells (CFs) as well as protein expression, alignment, and contractile function of cardiomyocyte (CMs) on PGS:gelatin scaffolds with variable amount of PGS. Notably, aligned nanofibrous scaffold, consisting of 33wt. % PGS, induced optimal synchronous contractions of CMs while significantly enhanced cellular alignment. Overall, our study suggests that the aligned nanofibrous PGS:gelatin scaffold support cardiac cell organization, phenotype and contraction and could potentially be used to develop clinically relevant constructs for cardiac tissue engineering.
AB - A significant challenge in cardiac tissue engineering is the development of biomimetic grafts that can potentially promote myocardial repair and regeneration. A number of approaches have used engineered scaffolds to mimic the architecture of the native myocardium tissue and precisely regulate cardiac cell functions. However, previous attempts have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the myocardial extracellular matrix (ECM). In this study, we utilized an electrospinning approach to fabricate elastomeric biodegradable poly(glycerol sebacate) (PGS):gelatin nanofibrous scaffolds with a wide range of chemical composition, stiffness and anisotropy. Our findings demonstrated that through incorporation of PGS, it is possible to create nanofibrous scaffolds with well-defined anisotropy that mimic the left ventricular myocardium architecture. Furthermore, we studied attachment, proliferation, differentiation and alignment of neonatal rat cardiac fibroblast cells (CFs) as well as protein expression, alignment, and contractile function of cardiomyocyte (CMs) on PGS:gelatin scaffolds with variable amount of PGS. Notably, aligned nanofibrous scaffold, consisting of 33wt. % PGS, induced optimal synchronous contractions of CMs while significantly enhanced cellular alignment. Overall, our study suggests that the aligned nanofibrous PGS:gelatin scaffold support cardiac cell organization, phenotype and contraction and could potentially be used to develop clinically relevant constructs for cardiac tissue engineering.
KW - Cardiac cells
KW - Nanofibrous
KW - Poly(glycerol sebacate):gelatin
KW - Scaffold
KW - Tissue engineering
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U2 - 10.1016/j.biomaterials.2013.04.045
DO - 10.1016/j.biomaterials.2013.04.045
M3 - Article
C2 - 23747008
AN - SCOPUS:84879080205
SN - 0142-9612
VL - 34
SP - 6355
EP - 6366
JO - Biomaterials
JF - Biomaterials
IS - 27
ER -