Abstract

We used a hydrogen (H2)-based biofilm to treat a groundwater contaminated with perchlorate (ClO4 -) at ∼10mg/L, an unusually high concentration. To enhance ClO4 - removal, we either increased the H2 pressure or decreased the electron-acceptor surface loading. The ClO4 - removal increased from 94% to 98% when the H2 pressure was increased from 1.3 to 1.7atm when the total acceptor surface loading was 0.49gH2/m2day. We then decreased the acceptor surface loading stepwise from 0.49 to 0.07gH2/m2day, and the ClO4 - removal improved to 99.6%, giving an effluent ClO4 - concentration of 41μg/L. However, the tradeoff was that sulfate (SO4 2-) reduction occurred, reaching 85% conversion at the lowest acceptor surface loading (0.07gH2/m2day). In two steady states with the highest ClO4 - reduction, we assayed for the presence of perchlorate-reducing bacteria (PRB), denitrifying bacteria (DB), and sulfate-reducing bacteria (SRB) by quantitative polymerase chain reaction (qPCR) targeting characteristic reductases. The qPCR results documented competition between PRB and SRB for space within the biofilm. A simple model analysis for a steady-state biofilm suggests that competition from SRB pushed the PRB to locations having a higher detachment rate, which prevented them from driving the ClO4 - concentration below 41μg/L.

Original languageEnglish (US)
Pages (from-to)3139-3147
Number of pages9
JournalBiotechnology and Bioengineering
Volume110
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Groundwater
Biofilms
Sulfates
Hydrogen
Bacteria
Polymerase chain reaction
perchlorate
Effluents
Pressure
Polymerase Chain Reaction
Electrons
Oxidoreductases

Keywords

  • Biofilm detachment
  • Groundwater remediation
  • Microbial community
  • Perchlorate reduction
  • QPCR
  • Sulfate reduction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Perchlorate reduction from a highly contaminated groundwater in the presence of sulfate-reducing bacteria in a hydrogen-fed biofilm. / Ontiveros-Valencia, Aura; Tang, Youneng; Krajmalnik-Brown, Rosa; Rittmann, Bruce.

In: Biotechnology and Bioengineering, Vol. 110, No. 12, 12.2013, p. 3139-3147.

Research output: Contribution to journalArticle

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