@article{877be49889f14545b2d61a7770c0b394,
title = "PDJ amplicon in triple negative breast cancer",
abstract = "Amplification of chromosome 9p24.1 targeting PD-L1, PD-L2, and JAK2 (PDJ amplicon) is present in subsets of triple negative breast cancers (TNBCs) and is associated with poor clinical outcomes. However, the prevalence of PDJ+ TNBCs varies extensively across studies applying different methods for interrogating samples of interest. To rigorously assess the prevalence of PDJ amplicons in TNBC, its prognostic value and whether it is enriched by chemotherapy, we interrogated 360 TNBC samples including 74 surgical resections from patients treated in the neoadjuvant setting, and tissue microarrays (TMAs) with 31 cases from African American women and 255 resected non-metastatic cases, with a 3 color fluorescence in situ hybridization (FISH) assay targeting the 9p24.1 PDJ amplicon, 9q24.3, and 9q34.1. Samples with mean PDJ signal of > 4.5 copies, and ratios of PDJ/9q24 ≥ 2 and/or PDJ/9q34.1 ≥ 2 were called amplified (PDJ+). Correlative analyses included the association of tumor infiltrating lymphocytes (TILs) with PDJ amplicons in TNBCs. In addition, we investigated intratumor copy number of PDJ amplicons in PDJ+ and PDJ− TNBCs. Matched pre- and post-neoadjuvant treatment biopsies were available from patients (n = 6) to evaluate the effects of therapy on PDJ status. Our study provides a rigorous analysis of the prevalence, distribution, and clinical correlatives of the PDJ amplicon in TNBC.",
author = "Roesler, {Alexander S.} and Smriti Malasi and Lori Koslosky and Peter Hartmayer and Naab, {Tammey J.} and Carter, {Jodi M.} and David Zahrieh and David Hillman and Leon-Ferre, {Roberto A.} and Couch, {Fergus J.} and Goetz, {Matthew P.} and Anderson, {Karen S.} and Pockaj, {Barbara A.} and Barrett, {Michael T.}",
note = "Funding Information: We thank the Mayo Clinic Cancer Center for the use of the Cytogenetics Core, which provided FISH services. The Mayo Cytogenetics Core, including Sara Kloft-Nelson, Darlene Knutson and Ryan Knudson, and the director, Patricia T. Greipp, provided excellent technical support for our study. The Mayo Clinic Cancer Center is supported in part by an NCI Cancer Center Support Grant (5P30 CA15083-36). Funding Information: We thank the Mayo Clinic Cancer Center for the use of the Cytogenetics Core, which provided FISH services. The Mayo Cytogenetics Core, including Sara Kloft-Nelson, Darlene Knutson and Ryan Knudson, and the director, Patricia T. Greipp, provided excellent technical support for our study. The Mayo Clinic Cancer Center is supported in part by an NCI Cancer Center Support Grant (5P30 CA15083-36). Funding Information: This work was supported by the Zicarelli Foundation (A.R. K.S.A. and B.A.P), the Breast Cancer Research Foundation (K.S.A.), and the Mayo Clinic Breast Cancer Specialized Program of Research Excellence (SPORE) (P50CA116201) (J.M.C. D.Z. R.L.F. F.J.C. and M.P.G). M.T.B. received funding through Mayo Clinic Center for Individualized Medicine Precision Cancer Therapeutics Program. Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
month = dec,
doi = "10.1038/s41598-023-27887-8",
language = "English (US)",
volume = "13",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}