PCR primers to amplify 16S rRNA genes from cyanobacteria

Ulrich Nübel, Ferran Garcia-Pichel, Gerard Muyzer

Research output: Contribution to journalArticle

914 Citations (Scopus)

Abstract

We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaei. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.

Original languageEnglish (US)
Pages (from-to)3327-3332
Number of pages6
JournalApplied and Environmental Microbiology
Volume63
Issue number8
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Cyanobacteria
rRNA Genes
cyanobacterium
Diatoms
Lichens
Bacillariophyceae
ribosomal RNA
lichen
Polymerase Chain Reaction
lichens
gene
diatom
Denaturing Gradient Gel Electrophoresis
Plastids
DNA Primers
genes
plastid
DNA primers
Molecular Cloning
denaturing gradient gel electrophoresis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

PCR primers to amplify 16S rRNA genes from cyanobacteria. / Nübel, Ulrich; Garcia-Pichel, Ferran; Muyzer, Gerard.

In: Applied and Environmental Microbiology, Vol. 63, No. 8, 08.1997, p. 3327-3332.

Research output: Contribution to journalArticle

Nübel, Ulrich ; Garcia-Pichel, Ferran ; Muyzer, Gerard. / PCR primers to amplify 16S rRNA genes from cyanobacteria. In: Applied and Environmental Microbiology. 1997 ; Vol. 63, No. 8. pp. 3327-3332.
@article{c5c4e03b90834dcd9595075d3f681f1d,
title = "PCR primers to amplify 16S rRNA genes from cyanobacteria",
abstract = "We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaei. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.",
author = "Ulrich N{\"u}bel and Ferran Garcia-Pichel and Gerard Muyzer",
year = "1997",
month = "8",
language = "English (US)",
volume = "63",
pages = "3327--3332",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - PCR primers to amplify 16S rRNA genes from cyanobacteria

AU - Nübel, Ulrich

AU - Garcia-Pichel, Ferran

AU - Muyzer, Gerard

PY - 1997/8

Y1 - 1997/8

N2 - We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaei. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.

AB - We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaei. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.

UR - http://www.scopus.com/inward/record.url?scp=0343247819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343247819&partnerID=8YFLogxK

M3 - Article

C2 - 9251225

AN - SCOPUS:0343247819

VL - 63

SP - 3327

EP - 3332

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -