PAX7+ satellite cells in young and older adults following resistance exercise

Dillon K. Walker; Christopher S. Fry; Micah J. Drummond; Jared M. Dickinson; Kyle L. Timmerman; David M. Gundermann; Kristofer Jennings; Elena Volpi; Blake B. Rasmussen

doi:10.1002/mus.23266

PAX7+ satellite cells in young and older adults following resistance exercise

Dillon K. Walker, Christopher S. Fry, Micah J. Drummond, Jared M. Dickinson, Kyle L. Timmerman, David M. Gundermann, Kristofer Jennings, Elena Volpi, Blake B. Rasmussen

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Introduction: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. Methods: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27^Kip1 and cyclin D1. Results: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27^Kip1. Conclusions: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.

Original language	English (US)
Pages (from-to)	51-59
Number of pages	9
Journal	Muscle and Nerve
Volume	46
Issue number	1
DOIs	https://doi.org/10.1002/mus.23266
State	Published - Jul 2012
Externally published	Yes

Keywords

Aging
Cell cycle
Gender
Muscle
Myonuclear domain

ASJC Scopus subject areas

Physiology
Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)

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title = "PAX7+ satellite cells in young and older adults following resistance exercise",

abstract = "Introduction: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. Methods: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27Kip1 and cyclin D1. Results: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27Kip1. Conclusions: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.",

keywords = "Aging, Cell cycle, Gender, Muscle, Myonuclear domain",

author = "Walker, {Dillon K.} and Fry, {Christopher S.} and Drummond, {Micah J.} and Dickinson, {Jared M.} and Timmerman, {Kyle L.} and Gundermann, {David M.} and Kristofer Jennings and Elena Volpi and Rasmussen, {Blake B.}",

year = "2012",

month = jul,

doi = "10.1002/mus.23266",

language = "English (US)",

volume = "46",

pages = "51--59",

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T1 - PAX7+ satellite cells in young and older adults following resistance exercise

AU - Walker, Dillon K.

AU - Fry, Christopher S.

AU - Drummond, Micah J.

AU - Dickinson, Jared M.

AU - Timmerman, Kyle L.

AU - Gundermann, David M.

AU - Jennings, Kristofer

AU - Volpi, Elena

AU - Rasmussen, Blake B.

PY - 2012/7

Y1 - 2012/7

N2 - Introduction: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. Methods: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27Kip1 and cyclin D1. Results: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27Kip1. Conclusions: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.

AB - Introduction: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. Methods: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27Kip1 and cyclin D1. Results: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27Kip1. Conclusions: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.

KW - Aging

KW - Cell cycle

KW - Gender

KW - Muscle

KW - Myonuclear domain

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PAX7+ satellite cells in young and older adults following resistance exercise

Abstract

Keywords

ASJC Scopus subject areas

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