PAX7+ satellite cells in young and older adults following resistance exercise

Dillon K. Walker, Christopher S. Fry, Micah J. Drummond, Jared M. Dickinson, Kyle L. Timmerman, David M. Gundermann, Kristofer Jennings, Elena Volpi, Blake B. Rasmussen

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Introduction: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. Methods: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27Kip1 and cyclin D1. Results: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27Kip1. Conclusions: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.

Original languageEnglish (US)
Pages (from-to)51-59
Number of pages9
JournalMuscle and Nerve
Volume46
Issue number1
DOIs
StatePublished - Jul 2012

Keywords

  • Aging
  • Cell cycle
  • Gender
  • Muscle
  • Myonuclear domain

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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    Walker, D. K., Fry, C. S., Drummond, M. J., Dickinson, J. M., Timmerman, K. L., Gundermann, D. M., Jennings, K., Volpi, E., & Rasmussen, B. B. (2012). PAX7+ satellite cells in young and older adults following resistance exercise. Muscle and Nerve, 46(1), 51-59. https://doi.org/10.1002/mus.23266