Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload

Jon-Philippe Hyatt, Gary E. McCall, Elizabeth M. Kander, Hui Zhong, Roland R. Roy, Kimberly A. Huey

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (≤7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self-renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental-like regulatory mechanisms in response to growth-inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells.

Original languageEnglish (US)
Pages (from-to)861-866
Number of pages6
JournalMuscle and Nerve
Volume38
Issue number1
DOIs
StatePublished - Jul 2008
Externally publishedYes

Fingerprint

Myogenic Regulatory Factors
Skeletal Muscle
PAX7 Transcription Factor
Messenger RNA
MyoD Protein
Muscle Development
Regulator Genes
Gene Expression
Growth
Genes

Keywords

  • Functional overload
  • Muscle regulatory factor
  • Plantaris
  • Satellite cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Hyatt, J-P., McCall, G. E., Kander, E. M., Zhong, H., Roy, R. R., & Huey, K. A. (2008). Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload. Muscle and Nerve, 38(1), 861-866. https://doi.org/10.1002/mus.21006

Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload. / Hyatt, Jon-Philippe; McCall, Gary E.; Kander, Elizabeth M.; Zhong, Hui; Roy, Roland R.; Huey, Kimberly A.

In: Muscle and Nerve, Vol. 38, No. 1, 07.2008, p. 861-866.

Research output: Contribution to journalArticle

Hyatt, J-P, McCall, GE, Kander, EM, Zhong, H, Roy, RR & Huey, KA 2008, 'Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload', Muscle and Nerve, vol. 38, no. 1, pp. 861-866. https://doi.org/10.1002/mus.21006
Hyatt, Jon-Philippe ; McCall, Gary E. ; Kander, Elizabeth M. ; Zhong, Hui ; Roy, Roland R. ; Huey, Kimberly A. / Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload. In: Muscle and Nerve. 2008 ; Vol. 38, No. 1. pp. 861-866.
@article{eab77421015a421badc1a9bde1970622,
title = "Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload",
abstract = "Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (≤7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self-renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental-like regulatory mechanisms in response to growth-inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells.",
keywords = "Functional overload, Muscle regulatory factor, Plantaris, Satellite cell",
author = "Jon-Philippe Hyatt and McCall, {Gary E.} and Kander, {Elizabeth M.} and Hui Zhong and Roy, {Roland R.} and Huey, {Kimberly A.}",
year = "2008",
month = "7",
doi = "10.1002/mus.21006",
language = "English (US)",
volume = "38",
pages = "861--866",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Pax3/7 expression coincides with MyoD during chronic skeletal muscle overload

AU - Hyatt, Jon-Philippe

AU - McCall, Gary E.

AU - Kander, Elizabeth M.

AU - Zhong, Hui

AU - Roy, Roland R.

AU - Huey, Kimberly A.

PY - 2008/7

Y1 - 2008/7

N2 - Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (≤7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self-renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental-like regulatory mechanisms in response to growth-inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells.

AB - Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (≤7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self-renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental-like regulatory mechanisms in response to growth-inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells.

KW - Functional overload

KW - Muscle regulatory factor

KW - Plantaris

KW - Satellite cell

UR - http://www.scopus.com/inward/record.url?scp=46249133974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46249133974&partnerID=8YFLogxK

U2 - 10.1002/mus.21006

DO - 10.1002/mus.21006

M3 - Article

C2 - 18508329

AN - SCOPUS:46249133974

VL - 38

SP - 861

EP - 866

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 1

ER -