TY - GEN
T1 - Pattern generation of a microfluidic-based biosensor to detect C-reactive protein using the competitive protein adsorption
AU - Choi, S.
AU - Lajevardi-Khosh, A.
AU - Chae, Junseok
PY - 2011
Y1 - 2011
N2 - We report a microfluidic-based protein sensing technique using pattern generation of a mixture of multiple proteins based on the competitive protein adsorption. We prepared four pre-adsorbed surfaces: lysozyme, albumin, transferrin, and IgG, on a gold surface, to obtain a selective and sensitive detection of a target protein, C-reactive protein (CRP) in a controlled cocktail. The microfluidic device was coupled with a Surface Plasmon Resonance (SPR), to monitor molecular reaction in real time. A matrix of proteins mixtures (1-antitrypsin, haptoglobin, CRP, and IgM) was generated to characterize the biosensor. Each surface interacted differently with a given sample and as a set generated a specific pattern based on individual SPR angle shifts. Using Linear Discriminant Analysis (LDA), we classified each protein and identified CRP selectively with an identification accuracy of 95 %.
AB - We report a microfluidic-based protein sensing technique using pattern generation of a mixture of multiple proteins based on the competitive protein adsorption. We prepared four pre-adsorbed surfaces: lysozyme, albumin, transferrin, and IgG, on a gold surface, to obtain a selective and sensitive detection of a target protein, C-reactive protein (CRP) in a controlled cocktail. The microfluidic device was coupled with a Surface Plasmon Resonance (SPR), to monitor molecular reaction in real time. A matrix of proteins mixtures (1-antitrypsin, haptoglobin, CRP, and IgM) was generated to characterize the biosensor. Each surface interacted differently with a given sample and as a set generated a specific pattern based on individual SPR angle shifts. Using Linear Discriminant Analysis (LDA), we classified each protein and identified CRP selectively with an identification accuracy of 95 %.
KW - Biosensor
KW - C-reactive protein
KW - Linear Discriminant Analysis (LDA)
KW - Microfluidics
KW - Pattern generation
KW - Surface Plasmon Resonance (SPR)
UR - http://www.scopus.com/inward/record.url?scp=80052111821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052111821&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969334
DO - 10.1109/TRANSDUCERS.2011.5969334
M3 - Conference contribution
AN - SCOPUS:80052111821
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 2223
EP - 2226
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -