Pattern generation of a microfluidic-based biosensor to detect C-reactive protein using the competitive protein adsorption

S. Choi, A. Lajevardi-Khosh, Junseok Chae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We report a microfluidic-based protein sensing technique using pattern generation of a mixture of multiple proteins based on the competitive protein adsorption. We prepared four pre-adsorbed surfaces: lysozyme, albumin, transferrin, and IgG, on a gold surface, to obtain a selective and sensitive detection of a target protein, C-reactive protein (CRP) in a controlled cocktail. The microfluidic device was coupled with a Surface Plasmon Resonance (SPR), to monitor molecular reaction in real time. A matrix of proteins mixtures (1-antitrypsin, haptoglobin, CRP, and IgM) was generated to characterize the biosensor. Each surface interacted differently with a given sample and as a set generated a specific pattern based on individual SPR angle shifts. Using Linear Discriminant Analysis (LDA), we classified each protein and identified CRP selectively with an identification accuracy of 95 %.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages2223-2226
Number of pages4
DOIs
StatePublished - Sep 1 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period6/5/116/9/11

Keywords

  • Biosensor
  • C-reactive protein
  • Linear Discriminant Analysis (LDA)
  • Microfluidics
  • Pattern generation
  • Surface Plasmon Resonance (SPR)

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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