Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys

Parag Chary, Silke Rautenschlein, M. K. Njenga, Jagdev M. Sharma

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Avian pneumovirus (APV) causes a respiratory disease in turkeys. The virus has been associated with morbidity and mortality due to secondary infections. Our objective was to determine if APV caused immunosuppression in the T-cell or B-cell compartments and to study the pathogenesis of the disease in APV maternal antibody-lacking 2-wk-old commercial turkeys. APV was administered by the eyedrop/intranasal route. Observations were made for gross lesions, viral genome, and T-cell mitogenesis and cytokine secretion at 3, 5, 7, 14, and 21 days postinoculation (DPI). During the acute phase of the disease that lasted for about 1 wk, the turkeys exposed to APV showed clinical signs characterized by nasal discharge and sinus swelling. Virus genome was detected by in situ hybridization in cells of turbinates and trachea at 3 and 5 DPI. At 3 and 5 DPI, spleen cells of the birds infected with APV markedly decreased proliferative response to concanavalin A (Con A). Con A and lipopolysaccharide stimulation of spleen cells from virus-exposed turkeys resulted in accumulation of nitric oxide-inducing factors (NOIF) in the culture fluid. NOIF were not detected in culture fluids of Con A-stimulated spleen cells of virus-free turkeys. APV did not compromise the antibody-producing ability of turkeys against several extraneous antigens such as Brucella abortus and tetanus toxoid.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalAvian Diseases
Volume46
Issue number1
StatePublished - 2002
Externally publishedYes

Fingerprint

Metapneumovirus
Avian metapneumovirus
immunosuppressive agents
Immunosuppressive Agents
concanavalin A
Concanavalin A
splenocytes
Viruses
viruses
Spleen
nitric oxide
Nitric Oxide
T-lymphocytes
T-Lymphocytes
Brucella abortus
Turbinates
trachea (vertebrates)
Tetanus Toxoid
toxoids
genome

Keywords

  • Avian metapneumovirus
  • Turkey
  • Turkey rhinotracheitis virus

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Chary, P., Rautenschlein, S., Njenga, M. K., & Sharma, J. M. (2002). Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys. Avian Diseases, 46(1), 153-161.

Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys. / Chary, Parag; Rautenschlein, Silke; Njenga, M. K.; Sharma, Jagdev M.

In: Avian Diseases, Vol. 46, No. 1, 2002, p. 153-161.

Research output: Contribution to journalArticle

Chary, P, Rautenschlein, S, Njenga, MK & Sharma, JM 2002, 'Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys', Avian Diseases, vol. 46, no. 1, pp. 153-161.
Chary P, Rautenschlein S, Njenga MK, Sharma JM. Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys. Avian Diseases. 2002;46(1):153-161.
Chary, Parag ; Rautenschlein, Silke ; Njenga, M. K. ; Sharma, Jagdev M. / Pathogenic and immunosuppressive effects of avian pneumovirus in turkeys. In: Avian Diseases. 2002 ; Vol. 46, No. 1. pp. 153-161.
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