Pathogenesis of type II avian adenovirus infection in Turkeys: In vivo immune cell tropism and tissue distribution of the virus

M. Suresh, J. M. Sharma

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Hemorrhagic enteritis virus (HEV), a type II avian adenovirus, causes intestinal hemorrhages and immunosuppression in turkeys. In this study, we exposed turkeys to virulent HEV and examined fractionated spleen cells for the presence of viral DNA by in situ hybridization and amplification of DNA extracted from virus-infected cells by PCR. HEV replication was detected only in the immunoglobulin M-bearing B lymphocytes and macrophage-Iike cells but not in the CD4+ or CD8+ T lymphocytes. The inability to infect T cells distinguishes type II avian adenoviruses from lymphotropic mammalian adenoviruses which infect and replicate in T cells. Furthermore, these data suggested that HEV-induced immunosuppression in turkeys may be due to the effect of the virus on B lymphocytes and macrophages. We also examined tissue tropism of HEV by in situ hybridization conducted on sections of lymphoid and nonlymphoid tissues. Large numbers of HEV-positive cells were detected in spleen and cecal tonsils. Diminutive viral activity was present in the intestines, the principal site of HEV-induced pathology. Thus, intestinal pathology was not associated with local cytopathic viral replication. This result and our previous observation that cyclosporin A abrogated intestinal hemorrhaging in HEV-infected turkeys strongly suggested that intestinal lesion induced by this virus may be immune system mediated.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalJournal of Virology
Volume70
Issue number1
StatePublished - 1996
Externally publishedYes

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Aviadenovirus
Adenoviridae Infections
tropisms
Tropism
tissue distribution
Tissue Distribution
Enteritis
pathogenesis
Viruses
viruses
Adenoviridae
cells
T-lymphocytes
immunosuppression
virus replication
T-Lymphocytes
Immunosuppression
in situ hybridization
B-lymphocytes
In Situ Hybridization

ASJC Scopus subject areas

  • Immunology

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Pathogenesis of type II avian adenovirus infection in Turkeys : In vivo immune cell tropism and tissue distribution of the virus. / Suresh, M.; Sharma, J. M.

In: Journal of Virology, Vol. 70, No. 1, 1996, p. 30-36.

Research output: Contribution to journalArticle

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