Abstract

Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.

Original languageEnglish (US)
Article numbere111673
JournalPLoS One
Volume9
Issue number11
DOIs
StatePublished - Nov 5 2014

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Plasma (human)
Pathogens
Pulsed lasers
Viruses
lasers
Lasers
Hepatitis A virus
pathogens
coagulation
Blood Coagulation Factors
Human herpesvirus 5
vertebrate viruses
Blood Safety
Human immunodeficiency virus
Muromegalovirus
fibrinogen
inactivation
methodology
adverse effects
Fibrinogen

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pathogen reduction in human plasma using an ultrashort pulsed laser. / Tsen, Shaw Wei D; Kingsley, David H.; Kibler, Karen; Jacobs, Bertram; Sizemore, Sara; Vaiana, Sara; Anderson, Jeanne; Tsen, Kong-Thon; Achilefu, Samuel.

In: PLoS One, Vol. 9, No. 11, e111673, 05.11.2014.

Research output: Contribution to journalArticle

Tsen, Shaw Wei D ; Kingsley, David H. ; Kibler, Karen ; Jacobs, Bertram ; Sizemore, Sara ; Vaiana, Sara ; Anderson, Jeanne ; Tsen, Kong-Thon ; Achilefu, Samuel. / Pathogen reduction in human plasma using an ultrashort pulsed laser. In: PLoS One. 2014 ; Vol. 9, No. 11.
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