Parallel synthesis of poly(amino ether)-templated plasmonic nanoparticles for transgene delivery

James Ramos, Thrimoorthy Potta, Olivia Scheideler, Kaushal Rege

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Plasmonic nanoparticles have been increasingly investigated for numerous applications in medicine, sensing, and catalysis. In particular, gold nanoparticles have been investigated for separations, sensing, drug/nucleic acid delivery, and bioimaging. In addition, silver nanoparticles demonstrate antibacterial activity, resulting in potential application in treatments against microbial infections, burns, diabetic skin ulcers, and medical devices. Here, we describe the facile, parallel synthesis of both gold and silver nanoparticles using a small set of poly(amino ethers), or PAEs, derived from linear polyamines, under ambient conditions and in absence of additional reagents. The kinetics of nanoparticle formation were dependent on PAE concentration and chemical composition. In addition, yields were significantly greater in case of PAEs when compared to 25 kDa poly(ethylene imine), which was used as a standard catonic polymer. Ultraviolet radiation enhanced the kinetics and the yield of both gold and silver nanoparticles, likely by means of a coreduction effect. PAE-templated gold nanoparticles demonstrated the ability to deliver plasmid DNA, resulting in transgene expression, in 22Rv1 human prostate cancer and MB49 murine bladder cancer cell lines. Taken together, our results indicate that chemically diverse poly(amino ethers) can be employed for rapidly templating the formation of metal nanoparticles under ambient conditions. The simplicity of synthesis and chemical diversity make PAE-templated nanoparticles useful tools for several applications in biotechnology, including nucleic acid delivery.

Original languageEnglish (US)
Pages (from-to)14861-14873
Number of pages13
JournalACS Applied Materials and Interfaces
Volume6
Issue number17
DOIs
StatePublished - Sep 10 2014

Keywords

  • combinatorial synthesis
  • gold nanoparticles
  • inorganic nanoparticles
  • nonviral gene delivery
  • photoreduction
  • silver nanoparticles
  • transfection

ASJC Scopus subject areas

  • Materials Science(all)

Fingerprint Dive into the research topics of 'Parallel synthesis of poly(amino ether)-templated plasmonic nanoparticles for transgene delivery'. Together they form a unique fingerprint.

Cite this