Abstract

Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device. Here, we present a simple, effective configuration for rapidly obtaining purified RNA from low concentration cell medium. This Total RNA Extraction Droplet Array (TREDA) utilizes an array of surface-adhering droplets to facilitate the transportation of magnetic purification beads seamlessly through individual buffer solutions without solid structures. The fabrication of TREDA chips is rapid and does not require a microfabrication facility or expertise. The process takes less than 5 minutes. When purifying mRNA from bulk marine diatom samples, its repeatability and extraction efficiency are comparable to conventional tube-based operations. We demonstrate that TREDA can extract the total mRNA of about 10 marine diatom cells, indicating that the sensitivity of TREDA approaches single-digit cell numbers.

Original languageEnglish (US)
Pages (from-to)1059-1065
Number of pages7
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number4
DOIs
StatePublished - Feb 21 2015

Fingerprint

RNA
Diatoms
Microtechnology
Messenger RNA
Lab-on-a-chip
Microfabrication
Nucleic acids
Nucleic Acids
Buffers
Cell Count
Purification
Solid solutions
Equipment and Supplies
Fabrication

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Parallel RNA extraction using magnetic beads and a droplet array. / Shi, Xu; Chen, Chun Hong; Gao, Weimin; Chao, Shih-Hui; Meldrum, Deirdre.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 15, No. 4, 21.02.2015, p. 1059-1065.

Research output: Contribution to journalArticle

@article{e369b81e0c1c47429e43cc4b84009dfe,
title = "Parallel RNA extraction using magnetic beads and a droplet array",
abstract = "Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device. Here, we present a simple, effective configuration for rapidly obtaining purified RNA from low concentration cell medium. This Total RNA Extraction Droplet Array (TREDA) utilizes an array of surface-adhering droplets to facilitate the transportation of magnetic purification beads seamlessly through individual buffer solutions without solid structures. The fabrication of TREDA chips is rapid and does not require a microfabrication facility or expertise. The process takes less than 5 minutes. When purifying mRNA from bulk marine diatom samples, its repeatability and extraction efficiency are comparable to conventional tube-based operations. We demonstrate that TREDA can extract the total mRNA of about 10 marine diatom cells, indicating that the sensitivity of TREDA approaches single-digit cell numbers.",
author = "Xu Shi and Chen, {Chun Hong} and Weimin Gao and Shih-Hui Chao and Deirdre Meldrum",
year = "2015",
month = "2",
day = "21",
doi = "10.1039/c4lc01111b",
language = "English (US)",
volume = "15",
pages = "1059--1065",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "4",

}

TY - JOUR

T1 - Parallel RNA extraction using magnetic beads and a droplet array

AU - Shi, Xu

AU - Chen, Chun Hong

AU - Gao, Weimin

AU - Chao, Shih-Hui

AU - Meldrum, Deirdre

PY - 2015/2/21

Y1 - 2015/2/21

N2 - Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device. Here, we present a simple, effective configuration for rapidly obtaining purified RNA from low concentration cell medium. This Total RNA Extraction Droplet Array (TREDA) utilizes an array of surface-adhering droplets to facilitate the transportation of magnetic purification beads seamlessly through individual buffer solutions without solid structures. The fabrication of TREDA chips is rapid and does not require a microfabrication facility or expertise. The process takes less than 5 minutes. When purifying mRNA from bulk marine diatom samples, its repeatability and extraction efficiency are comparable to conventional tube-based operations. We demonstrate that TREDA can extract the total mRNA of about 10 marine diatom cells, indicating that the sensitivity of TREDA approaches single-digit cell numbers.

AB - Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device. Here, we present a simple, effective configuration for rapidly obtaining purified RNA from low concentration cell medium. This Total RNA Extraction Droplet Array (TREDA) utilizes an array of surface-adhering droplets to facilitate the transportation of magnetic purification beads seamlessly through individual buffer solutions without solid structures. The fabrication of TREDA chips is rapid and does not require a microfabrication facility or expertise. The process takes less than 5 minutes. When purifying mRNA from bulk marine diatom samples, its repeatability and extraction efficiency are comparable to conventional tube-based operations. We demonstrate that TREDA can extract the total mRNA of about 10 marine diatom cells, indicating that the sensitivity of TREDA approaches single-digit cell numbers.

UR - http://www.scopus.com/inward/record.url?scp=84922495972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922495972&partnerID=8YFLogxK

U2 - 10.1039/c4lc01111b

DO - 10.1039/c4lc01111b

M3 - Article

VL - 15

SP - 1059

EP - 1065

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 4

ER -