Abstract

Scaffolds generated from naturally occurring and synthetic polymers have been investigated in several applications because of their biocompatibility and tunable chemo-mechanical properties. Existing methods for generation of 3D polymeric scaffolds typically cannot be parallelized, suffer from low throughputs, and do not allow for quick and easy removal of the fragile structures that are formed. Current molds used in hydrogel and scaffold fabrication using solvent casting and porogen leaching are often single-use and do not facilitate 3D scaffold formation in parallel. Here, we describe a simple device and related approaches for the parallel fabrication of macroporous scaffolds. This approach was employed for the generation of macroporous and non-macroporous materials in parallel, in higher throughput and allowed for easy retrieval of these 3D scaffolds once formed. In addition, macroporous scaffolds with interconnected as well as non-interconnected pores were generated, and the versatility of this approach was employed for the generation of 3D scaffolds from diverse materials including an aminoglycoside-derived cationic hydrogel ("Amikagel"), poly(lactic-co-glycolic acid) or PLGA, and collagen. Macroporous scaffolds generated using the device were investigated for plasmid DNA binding and cell loading, indicating the use of this approach for developing materials for different applications in biotechnology. Our results demonstrate that the device-based approach is a simple technology for generating scaffolds in parallel, which can enhance the toolbox of current fabrication techniques.

Original languageEnglish (US)
JournalBiotechnology and Bioengineering
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Scaffolds
Hydrogel
Fabrication
Equipment and Supplies
Aminoglycosides
Biotechnology
Polymers
Plasmids
Fungi
Collagen
Technology
Hydrogels
DNA
Throughput
Molds
Scaffolds (biology)
Biocompatibility
Leaching
polylactic acid-polyglycolic acid copolymer
Casting

Keywords

  • Biomaterials
  • Plasmid DNA
  • Polymeric scaffolds

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Parallel fabrication of macroporous scaffolds. / Dobos, Andrew; Grandhi, Taraka Sai Pavan; Godeshala, Sudhakar; Meldrum, Deirdre; Rege, Kaushal.

In: Biotechnology and Bioengineering, 01.01.2018.

Research output: Contribution to journalArticle

Dobos, Andrew ; Grandhi, Taraka Sai Pavan ; Godeshala, Sudhakar ; Meldrum, Deirdre ; Rege, Kaushal. / Parallel fabrication of macroporous scaffolds. In: Biotechnology and Bioengineering. 2018.
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